PRKRA (S246) Blocking Peptide | PRKRA blocking peptide

PRKRA Antibody (S246) Blocking Peptide

• Gene Names: PRKRA; RAX; PACT; DYT16; HSD14
• Synonyms: PRKRA (S246); PRKRA Antibody (S246) Blocking Peptide; Interferon-inducible double-stranded RNA-dependent protein kinase activator A; PKR-associated protein X; PKR-associating protein X; Protein activator of the interferon-induced protein kinase; Protein kinase; interferon-inducible double-stranded RNA-dependent activator; PRKRA; PACT; RAX; PRKRA blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the S246 region of human PRKRA. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 313

• Cellular Location: Cytoplasm, perinuclear region. Cytoplasm.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PRKRA blocking peptide

Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post- transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 74735517
• NCBI GeneID: 8575
• UniProt Accession #: O75569; Q53G24; Q6X7T5; Q8NDK4; A8K3I6
• Molecular Weight: 31,635 Da
• NCBI Official Full Name: Interferon-inducible double-stranded RNA-dependent protein kinase activator A
• NCBI Official Synonym Full Names: protein activator of interferon induced protein kinase EIF2AK2
• NCBI Official Symbol: PRKRA
• NCBI Official Synonym Symbols: RAX; PACT; DYT16; HSD14
• NCBI Protein Information: interferon-inducible double-stranded RNA-dependent protein kinase activator A
• UniProt Protein Name: Interferon-inducible double-stranded RNA-dependent protein kinase activator A
• Protein Family: Interferon-inducible double-stranded RNA-dependent protein kinase activator
• UniProt Gene Name: PRKRA
• UniProt Synonym Gene Names: PACT; RAX
• UniProt Entry Name: PRKRA_HUMAN

NCBI Description

This gene encodes a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]

Uniprot Description

PACT: a double strand RNA binding protein (dsRBP). Interacts with TRBP and Dicer to stimulate the cleavage of double-stranded or short hairpin RNA to siRNA. Appears to have a pro-apoptotic function that may be suppressed in the presence of growth factor. Activates the interferon-induced protein kinase PKR. Three alternatively spliced human isoforms have been reported.

Protein type: RNA-binding; Activator; RNA processing

Chromosomal Location of Human Ortholog: 2q31.2

Cellular Component: cytoplasm; cytosol; membrane; nucleoplasm

Molecular Function: double-stranded RNA binding; enzyme binding; identical protein binding; protein binding; protein homodimerization activity

Biological Process: immune response; miRNA-mediated gene silencing, production of miRNAs; negative regulation of cell proliferation; pre-microRNA processing; response to virus; RNA interference, production of siRNA; RNA-mediated gene silencing

Disease: Dystonia 16