PPP1R9B blocking peptide

PPP1R9B Antibody (N-term) Blocking Peptide

• Gene Names: PPP1R9B; Spn; SPINO; PPP1R6; PPP1R9
• Synonyms: PPP1R9B; PPP1R9B Antibody (N-term) Blocking Peptide; Neurabin-2; Neurabin-II; Protein phosphatase 1 regulatory subunit 9B; Spinophilin; PPP1R6; PPP1R9B blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 815

• Cellular Location: Cytoplasm, cytoskeleton. Nucleus. Cell projection, dendritic spine. Cell junction, synapse. Cell junction, adherens junction. Cytoplasm. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium. Cell projection, ruffle membrane. Note: Enriched at synapse and cadherin-based cell- cell adhesion sites. In neurons, both cytosolic and membrane- associated, and highly enriched in the postsynaptic density apposed to exitatory synapses. Colocalizes with PPP1R2 at actin- rich adherens junctions in epithelial cells and in dendritic spines (By similarity). Accumulates in the lamellipodium, filopodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PPP1R9B blocking peptide

Seems to act as a scaffold protein in multiple signaling pathways. Modulates excitatory synaptic transmission and dendritic spine morphology. Binds to actin filaments (F-actin) and shows cross-linking activity. Binds along the sides of the F-actin. May play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. Believed to target protein phosphatase 1/PP1 to dendritic spines, which are rich in F-actin, and regulates its specificity toward ion channels and other substrates, such as AMPA-type and NMDA-type glutamate receptors. Plays a role in regulation of G-protein coupled receptor signaling, including dopamine D2 receptors and alpha- adrenergic receptors. May establish a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Binds to ADRA1B and RGS2 and mediates regulation of ADRA1B signaling. May confer to Rac signaling specificity by binding to both, RacGEFs and Rac effector proteins. Probably regulates p70 S6 kinase activity by forming a complex with TIAM1 (By similarity). Required for hepatocyte growth factor (HGF)- induced cell migration.

NCBI and Uniprot Product Information

• NCBI GI #: 90101416
• NCBI GeneID: 84687
• UniProt Accession #: Q96SB3; Q8TCR9
• Molecular Weight: 89,192 Da
• NCBI Official Full Name: Neurabin-2
• NCBI Official Synonym Full Names: protein phosphatase 1 regulatory subunit 9B
• NCBI Official Symbol: PPP1R9B
• NCBI Official Synonym Symbols: Spn; SPINO; PPP1R6; PPP1R9
• NCBI Protein Information: neurabin-2
• UniProt Protein Name: Neurabin-2
• Protein Family: Neurabin
• UniProt Gene Name: PPP1R9B
• UniProt Synonym Gene Names: PPP1R6
• UniProt Entry Name: NEB2_HUMAN

NCBI Description

This gene encodes a scaffold protein that functions as a regulatory subunit of protein phosphatase 1a. Expression of this gene is particularly high in dendritic spines, suggesting that the encoded protein may play a role in receiving signals from the central nervous system. The encoded protein has putative tumor suppressor function and decreased expression has been observed in tumors. [provided by RefSeq, Feb 2014]

Uniprot Description

Spinophilin: actin-binding regulatory subunit of protein phosphatase 1 (PP1). The actin-binding domain of spinophilin is necessary and sufficient for targeting of spinophilin to dendrites and dendritic spines. May play a role in establishing a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Interacts with p14-ARF. Contains one PDZ/DHR domain.

Protein type: Protein phosphatase, regulatory subunit; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q21.33

Cellular Component: actin cytoskeleton; cytoplasm; dendrite; filopodium; lamellipodium; nucleoplasm; plasma membrane; postsynaptic density; protein phosphatase type 1 complex

Molecular Function: actin filament binding; protein binding

Biological Process: actin filament organization; calcium-mediated signaling; cell cycle arrest; cell migration; filopodium formation; negative regulation of cell growth; neurite development; regulation of cell growth by extracellular stimulus