POLH blocking peptide

POLH Blocking Peptide

Cat. Num. AAA9626018

• Gene Names: POLH; XPV; XP-V; RAD30; RAD30A
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: POLH; POLH Blocking Peptide; DNA polymerase eta; FLJ16395; FLJ21978; Polh; POLH_HUMAN; polymerase DNA directed eta; RAD30; RAD30 homolog A; RAD30A; Xeroderma pigmentosum variant type protein; XP V; XPV; POLH blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for POLH blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for POLH blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 634743306
• NCBI GeneID: 5429
• NCBI Accession #: NP_001278899.1
• NCBI GenBank Nucleotide #: NM_001291970.1
• UniProt Accession #: Q9Y253; Q7L8E3; Q96BC4; Q9BX13
• Molecular Weight: 46,283 Da
• NCBI Official Full Name: DNA polymerase eta isoform 3
• NCBI Official Synonym Full Names: polymerase (DNA) eta
• NCBI Official Symbol: POLH
• NCBI Official Synonym Symbols: XPV; XP-V; RAD30; RAD30A
• NCBI Protein Information: DNA polymerase eta
• UniProt Protein Name: DNA polymerase eta
• Protein Family: DNA polymerase
• UniProt Gene Name: POLH
• UniProt Synonym Gene Names: RAD30; RAD30A; XPV
• UniProt Entry Name: POLH_HUMAN

NCBI Description

This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]

Uniprot Description

POLH: DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI to replication foci. Interacts with REV1. Interacts with monoubiquitinated PCNA, but not unmodified PCNA. Interacts with POLI. Belongs to the DNA polymerase type-Y family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; EC 2.7.7.7; DNA repair, damage

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding; metal ion binding; damaged DNA binding; DNA-directed DNA polymerase activity

Biological Process: regulation of DNA repair; DNA synthesis during DNA repair; postreplication repair; pyrimidine dimer repair; DNA repair; DNA replication; response to UV-C

Disease: Xeroderma Pigmentosum, Variant Type