PIM3 blocking peptide

PIM3 Antibody (C-term) Blocking Peptide

• Gene Names: PIM3; pim-3
• Synonyms: PIM3; PIM3 Antibody (C-term) Blocking Peptide; Serine/threonine-protein kinase pim-3; PIM3 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human PIM3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 326

• Cellular Location: Cytoplasm.
• Tissue Location: Detected in various tissues, including the heart, brain, lung, kidney, spleen, placenta, skeletal muscle, and peripheral blood leukocytes. Not found or barely expressed in the normal adult endoderm-derived organs such as colon, thymus, liver, or small intestine. However, expression is augmented in premalignant and malignant lesions of these organs
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PIM3 blocking peptide

Proto-oncogene with serine/threonine kinase activity that can prevent apoptosis, promote cell survival and protein translation. May contribute to tumorigenesis through: the delivery of survival signaling through phosphorylation of BAD which induces release of the anti-apoptotic protein Bcl-X(L), the regulation of cell cycle progression, protein synthesis and by regulation of MYC transcriptional activity. Additionally to this role on tumorigenesis, can also negatively regulate insulin secretion by inhibiting the activation of MAPK1/3 (ERK1/2), through SOCS6. Involved also in the control of energy metabolism and regulation of AMPK activity in modulating MYC and PPARGC1A protein levels and cell growth.

NCBI and Uniprot Product Information

• NCBI GI #: 215274221
• NCBI GeneID: 415116
• UniProt Accession #: Q86V86; Q68BM2; A5D8X8; A8K7J0; B1B0P0
• Molecular Weight: 35,891 Da
• NCBI Official Full Name: Serine/threonine-protein kinase pim-3
• NCBI Official Synonym Full Names: Pim-3 proto-oncogene, serine/threonine kinase
• NCBI Official Symbol: PIM3
• NCBI Official Synonym Symbols: pim-3
• NCBI Protein Information: serine/threonine-protein kinase pim-3
• UniProt Protein Name: Serine/threonine-protein kinase pim-3
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PIM3
• UniProt Entry Name: PIM3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is overexpressed in hematological and epithelial tumors and is associated with MYC coexpression. It plays a role in the regulation of signal transduction cascades, contributing to both cell proliferation and survival, and provides a selective advantage in tumorigenesis. [provided by RefSeq, Jun 2012]

Uniprot Description

Pim3: a Ca(2 )/calmodulin-dependent protein kinase of the PIM family. Highly expressed in hematopoietic tissues, in leukemia and lymphoma cell lines, testis, small intestine, colon and colorectal adenocarcinoma cells.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; Kinase, protein; Oncoprotein; CAMK group; PIM family

Chromosomal Location of Human Ortholog: 22q13

Cellular Component: cytoplasm

Molecular Function: protein binding; protein serine/threonine kinase activity

Biological Process: negative regulation of apoptosis; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of mitotic cell cycle