PI4K2A (PI4K II) Blocking Peptide | PI4K2A blocking peptide

PI4K2A (PI4K II) Antibody (N-term) Blocking peptide

• Gene Names: PI4K2A; PI4KII; PIK42A
• Synonyms: PI4K2A (PI4K II); PI4K2A (PI4K II) Antibody (N-term) Blocking peptide; Phosphatidylinositol 4-kinase type 2-alpha; Phosphatidylinositol 4-kinase type II-alpha; PI4K2A; PI4K2A blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human PI4K II. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 479

• Cellular Location: Golgi apparatus, trans-Golgi network membrane; Lipid- anchor. Membrane raft. Cell projection, dendrite. Cell junction, synapse, presynaptic cell membrane. Cell junction, synapse, synaptosome Mitochondrion. Endosome Cytoplasmic vesicle. Membrane; Lipid-anchor. Cell membrane Note: Localized in neuronal cell body. Transported from neuronal cell body to neuron projections in a BLOC-1- and AP-3-complexes- dependent manner. Enriched in neurite tips and neuron projections in a BLOC-1- and AP-3-complexes-dependent manner (By similarity) Found in subdomains of the plasma membrane termed non-caveolar membrane rafts.
• Tissue Location: Widely expressed. Highest expression is observed in kidney, brain, heart, skeletal muscle, and placenta and lowest expression is observed in colon, thymus, and small intestine.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PI4K2A blocking peptide

Membrane-bound phosphatidylinositol-4 kinase (PI4- kinase) that catalyzes the phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P), a lipid that plays important roles in endocytosis, Golgi function, protein sorting and membrane trafficking and is required for prolonged survival of neurons. Besides, phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P) is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3).

NCBI and Uniprot Product Information

• NCBI GI #: 74752344
• NCBI GeneID: 55361
• UniProt Accession #: Q9BTU6; Q9NSG8; D3DR59
• Molecular Weight: 54,022 Da
• NCBI Official Full Name: Phosphatidylinositol 4-kinase type 2-alpha
• NCBI Official Synonym Full Names: phosphatidylinositol 4-kinase type 2 alpha
• NCBI Official Symbol: PI4K2A
• NCBI Official Synonym Symbols: PI4KII; PIK42A
• NCBI Protein Information: phosphatidylinositol 4-kinase type 2-alpha
• UniProt Protein Name: Phosphatidylinositol 4-kinase type 2-alpha
• Protein Family: Phosphatidylinositol 4-kinase
• UniProt Gene Name: PI4K2A
• UniProt Entry Name: P4K2A_HUMAN

NCBI Description

Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs (Barylko et al., 2001 [PubMed 11244087]).[supplied by OMIM, Mar 2008]

Uniprot Description

PI4K2A: Together with PI4K2B and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells. Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.

Protein type: EC 2.7.1.67; Motility/polarity/chemotaxis; Kinase, lipid

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: cell soma; cytoplasmic vesicle; cytosol; dendrite; endosome; integral to plasma membrane; intrinsic to membrane; lipid raft; lysosomal membrane; membrane; mitochondrion; neuron projection; presynaptic membrane

Molecular Function: 1-phosphatidylinositol 4-kinase activity; ATP binding; protein binding

Biological Process: phosphatidylinositol biosynthetic process; phosphoinositide phosphorylation