PDPK1 (S396) Blocking Peptide | PDPK1 blocking peptide

Phospho-PDPK1(S396) Antibody Blocking peptide

• Gene Names: PDPK1; PDK1; PDPK2; PDPK2P; PRO0461
• Synonyms: PDPK1 (S396); Phospho-PDPK1(S396) Antibody Blocking peptide; 3-phosphoinositide-dependent protein kinase 1; hPDK1; PDPK1; PDK1; PDPK1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the region of human Phospho-PDPK1-S396. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 556

• Cellular Location: Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Cell junction, focal adhesion Note: Tyrosine phosphorylation seems to occur only at the cell membrane. Translocates to the cell membrane following insulin stimulation by a mechanism that involves binding to GRB14 and INSR. SRC and HSP90 promote its localization to the cell membrane Its nuclear localization is dependent on its association with PTPN6 and its phosphorylation at Ser-396. Restricted to the nucleus in neuronal cells while in non-neuronal cells it is found in the cytoplasm. The Ser-241 phosphorylated form is distributed along the perinuclear region in neuronal cells while in non- neuronal cells it is found in both the nucleus and the cytoplasm IGF1 transiently increases phosphorylation at Ser-241 of neuronal PDPK1, resulting in its translocation to other cellular compartments. The tyrosine-phosphorylated form colocalizes with PTK2B in focal adhesions after angiotensin II stimulation
• Tissue Location: Appears to be expressed ubiquitously. The Tyr- 9 phosphorylated form is markedly increased in diseased tissue compared with normal tissue from lung, liver, colon and breast
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PDPK1 blocking peptide

Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptional activity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controls proliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca(2+) entry and Ca(2+)-activated K(+) channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses. Provides negative feedback inhibition to toll-like receptor-mediated NF- kappa-B activation in macrophages. Isoform 3 is catalytically inactive.

NCBI and Uniprot Product Information

• NCBI GI #: 17380162
• NCBI GeneID: 5170
• UniProt Accession #: O15530; Q59EH6; Q6FI20; Q8IV52; Q9BRD5; H0Y4Z0; Q6A1A2
• Molecular Weight: 50,838 Da
• NCBI Official Full Name: 3-phosphoinositide-dependent protein kinase 1
• NCBI Official Synonym Full Names: 3-phosphoinositide dependent protein kinase 1
• NCBI Official Symbol: PDPK1
• NCBI Official Synonym Symbols: PDK1; PDPK2; PDPK2P; PRO0461
• NCBI Protein Information: 3-phosphoinositide-dependent protein kinase 1
• UniProt Protein Name: 3-phosphoinositide-dependent protein kinase 1
• Protein Family: 3-phosphoinositide-dependent protein kinase
• UniProt Gene Name: PDPK1
• UniProt Synonym Gene Names: PDK1; hPDK1
• UniProt Entry Name: PDPK1_HUMAN

Uniprot Description

PDK1: an AGC kinase of the PKB family that contains a PH domain. Involved in a wide variety of processes including cell proliferation, differentiation and apoptosis. Autophosphorylation in the activation loop is necessary for activity. Phosphorylates and activates kinases including Akt, PKC isozymes, p70 S6K and RSK. Three alternatively-spliced isoforms have been described.

Protein type: Nuclear receptor co-regulator; Protein kinase, AGC; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); AGC group; PKB family

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: cell projection; cytoplasm; cytosol; intracellular; nucleoplasm; plasma membrane

Molecular Function: 3-phosphoinositide-dependent protein kinase activity; kinase activity; phospholipase activator activity; phospholipase binding; protein binding; protein serine/threonine kinase activity

Biological Process: actin cytoskeleton organization and biogenesis; activation of protein kinase B; calcium-mediated signaling; cell migration; cellular response to insulin stimulus; epidermal growth factor receptor signaling pathway; negative regulation of protein kinase activity; negative regulation of transforming growth factor beta receptor signaling pathway; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphoinositide-mediated signaling; platelet activation; positive regulation of release of sequestered calcium ion into cytosol; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of I-kappaB kinase/NF-kappaB cascade; stimulatory C-type lectin receptor signaling pathway; T cell costimulation; T cell receptor signaling pathway