Rabbit PDPK1 Polyclonal Antibody | anti-PDPK1 antibody

PDPK1 Antibody

Cat. Num. AAA9407949

• Gene Names: PDPK1; PDK1; PDPK2; PDPK2P; PRO0461
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: PDPK1; Polyclonal Antibody; PDPK1 Antibody; PDK1; PDPK2; PRO0461; anti-PDPK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total PDPK1 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 454

• Applicable Applications for anti-PDPK1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant Protein of human PDPK1.
• Target Name: PDPK1
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PDPK1 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using PDPK1 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-PDPK1 antibody

Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptional activity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controls proliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca2+ entry and Ca2+-activated K+ channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses. Provides negative feedback inhibition to toll-like receptor-mediated NF-kappa-B activation in macrophages. Isoform 3is catalytically inactive.

Product Categories/Family for anti-PDPK1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 387849239
• NCBI GeneID: 5170
• NCBI Accession #: NP_001248745.1
• NCBI GenBank Nucleotide #: NM_001261816.1
• UniProt Accession #: O15530; Q59EH6; Q6FI20; Q8IV52; Q9BRD5; H0Y4Z0; Q6A1A2
• Molecular Weight: 50,838 Da
• NCBI Official Full Name: 3-phosphoinositide-dependent protein kinase 1 isoform 3
• NCBI Official Synonym Full Names: 3-phosphoinositide dependent protein kinase 1
• NCBI Official Symbol: PDPK1
• NCBI Official Synonym Symbols: PDK1; PDPK2; PDPK2P; PRO0461
• NCBI Protein Information: 3-phosphoinositide-dependent protein kinase 1
• UniProt Protein Name: 3-phosphoinositide-dependent protein kinase 1
• Protein Family: 3-phosphoinositide-dependent protein kinase
• UniProt Gene Name: PDPK1
• UniProt Synonym Gene Names: PDK1; hPDK1
• UniProt Entry Name: PDPK1_HUMAN

Uniprot Description

PDK1: an AGC kinase of the PKB family that contains a PH domain. Involved in a wide variety of processes including cell proliferation, differentiation and apoptosis. Autophosphorylation in the activation loop is necessary for activity. Phosphorylates and activates kinases including Akt, PKC isozymes, p70 S6K and RSK. Three alternatively-spliced isoforms have been described.

Protein type: Nuclear receptor co-regulator; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; PKB family

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; cell projection; focal adhesion; membrane; cytoplasmic membrane-bound vesicle; cytoplasm; plasma membrane; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; phospholipase binding; 3-phosphoinositide-dependent protein kinase activity; kinase activity; protein kinase binding; ATP binding; insulin receptor binding; phospholipase activator activity

Biological Process: focal adhesion formation; regulation of mast cell degranulation; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; T cell receptor signaling pathway; protein amino acid phosphorylation; synaptic transmission; regulation of transcription, DNA-dependent; regulation of I-kappaB kinase/NF-kappaB cascade; epidermal growth factor receptor signaling pathway; platelet activation; cell migration; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; transcription, DNA-dependent; activation of protein kinase B; peptidyl-threonine phosphorylation; calcium-mediated signaling; hyperosmotic response; cellular response to insulin stimulus; T cell costimulation; insulin receptor signaling pathway; negative regulation of toll-like receptor signaling pathway; innate immune response; negative regulation of protein kinase activity; positive regulation of release of sequestered calcium ion into cytosol; actin cytoskeleton organization and biogenesis; blood coagulation; negative regulation of transforming growth factor beta receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway