PDE1B blocking peptide

PDE1B Blocking Peptide

Cat. Num. AAA9626196

• Gene Names: PDE1B; PDE1B1; PDES1B; HEL-S-79p
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: PDE1B; PDE1B Blocking Peptide; 5''-cyclic nucleotide phosphodiesterase 1B; 63 kDa Cam PDE; 63 kDa Cam-PDE; Calcium/calmodulin dependent 3' 5' cyclic nucleotide Phosphodiesterase 1B; Calcium/calmodulin stimulated cyclic nucleotide phosphodiesterase; Calcium/calmodulin-dependent 3''; Calmodulin stimulated phosphodiesterase PDE1B1; Cam PDE 1B; Cam PDE1B; Cam-PDE 1B; PDE 1B; PDE1B_HUMAN; PDE1B1; PDES 1B; PDES1B; Phosphodiesterase 1B; Phosphodiesterase 1B calmodulin dependent; Presumed 63kDa form of the type 1 cyclic nucleotide phosphodiesterase family known as PDE1B; PDE1B blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for PDE1B blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PDE1B blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 4505677
• NCBI GeneID: 5153
• NCBI Accession #: NP_000915.1
• NCBI GenBank Nucleotide #: NM_000924.3
• UniProt Accession #: Q01064; Q92825; Q96KP3
• Molecular Weight: 59,051 Da
• NCBI Official Full Name: calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B isoform 1
• NCBI Official Synonym Full Names: phosphodiesterase 1B
• NCBI Official Symbol: PDE1B
• NCBI Official Synonym Symbols: PDE1B1; PDES1B; HEL-S-79p
• NCBI Protein Information: calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B
• UniProt Protein Name: Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B
• UniProt Gene Name: PDE1B
• UniProt Synonym Gene Names: PDE1B1; PDES1B; Cam-PDE 1B
• UniProt Entry Name: PDE1B_HUMAN

NCBI Description

The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]

Uniprot Description

PDE1B: Cyclic nucleotide phosphodiesterase with a dual- specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate. Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.17; Nucleotide Metabolism - purine; Phosphodiesterase; Apoptosis

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: cell soma; cytosol

Molecular Function: calmodulin binding; 3',5'-cyclic-AMP phosphodiesterase activity; calmodulin-dependent cyclic-nucleotide phosphodiesterase activity; calcium- and calmodulin-regulated 3',5'-cyclic-GMP phosphodiesterase activity; metal ion binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; monocyte differentiation; apoptosis; regulation of neurotransmitter levels; response to amphetamine; cAMP catabolic process; regulation of dopamine metabolic process; locomotory behavior; signal transduction; phospholipase C activation; innate immune response; visual learning; cGMP catabolic process; blood coagulation; serotonin metabolic process