PAPSS1 blocking peptide

PAPSS1 Antibody (N-term K9) Blocking Peptide

• Gene Names: PAPSS1; SK1; PAPSS; ATPSK1
• Synonyms: PAPSS1; PAPSS1 Antibody (N-term K9) Blocking Peptide; Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1; PAPS synthase 1; PAPSS 1; Sulfurylase kinase 1; SK 1; SK1; Sulfate adenylyltransferase; ATP-sulfurylase; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; 3'-phosphoadenosine-5'-phosphosulfate synthase; APS kinase; Adenosine-5'-phosphosulfate 3'-phosphotransferase; Adenylylsulfate 3'-phosphotransferase; ATPSK1; PAPSS; PAPSS1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human PAPSS1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 624

• Tissue Location: Expressed in testis, pancreas, kidney, thymus, prostate, ovary, small intestine, colon, leukocytes and liver Also expressed in high endothelial venules (HEV) cells and in cartilage
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PAPSS1 blocking peptide

Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate- activation pathway. Also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells.

NCBI and Uniprot Product Information

• NCBI GI #: 46094058
• NCBI GeneID: 9061
• NCBI Accession #: NP_005434
• NCBI GenBank Nucleotide #: NM_005443.4
• UniProt Accession #: O43252; O43841; O75332; Q96FB1; Q96TF4; Q9P1P9; Q9UE98
• Molecular Weight: 70,833 Da
• NCBI Official Full Name: bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
• NCBI Official Synonym Full Names: 3'-phosphoadenosine 5'-phosphosulfate synthase 1
• NCBI Official Symbol: PAPSS1
• NCBI Official Synonym Symbols: SK1; PAPSS; ATPSK1
• NCBI Protein Information: bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
• UniProt Protein Name: Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
• Protein Family: Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase
• UniProt Gene Name: PAPSS1
• UniProt Synonym Gene Names: ATPSK1; PAPSS; PAPS synthase 1; PAPSS 1; SK 1; SK1; SAT
• UniProt Entry Name: PAPS1_HUMAN

NCBI Description

Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).[supplied by OMIM, Mar 2008]

Uniprot Description

PAPSS1: Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate- activation pathway. Also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells.

Protein type: EC 2.7.7.4; Nucleotide Metabolism - purine; Energy Metabolism - sulfur; Kinase, other; Other Amino Acids Metabolism - selenoamino acid; EC 2.7.1.25

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: cytosol

Molecular Function: adenylylsulfate kinase activity; nucleotidyltransferase activity; sulfate adenylyltransferase (ATP) activity

Biological Process: 3'-phosphoadenosine 5'-phosphosulfate biosynthetic process; skeletal development; sulfate assimilation