OTUB2 blocking peptide

OTUB2 Blocking Peptide

Cat. Num. AAA9619644

• Gene Names: OTUB2; OTB2; OTU2; C14orf137
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: OTUB2; OTUB2 Blocking Peptide; C14orf137; Deubiquitinating enzyme OTUB2; FLJ21916; MGC3102; OTB2; OTU domain containing ubiquitin aldehyde binding protein 2; OTU domain; ubiquitin aldehyde binding 2; OTU domain-containing ubiquitin aldehyde-binding protein 2; OTU2; Otub2; OTUB2_HUMAN; Otubain 2; Otubain-2; Ubiquitin specific processing protease OTUB2; Ubiquitin specific protease otubain 2; Ubiquitin thioesterase OTUB2; Ubiquitin-specific-processing protease OTUB2; OTUB2 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for OTUB2 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for OTUB2 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 12962939
• NCBI GeneID: 78990
• NCBI Accession #: NP_075601.1
• NCBI GenBank Nucleotide #: NM_023112.3
• UniProt Accession #: Q96DC9; Q6IA10; Q9H6T1
• Molecular Weight: 8,637 Da
• NCBI Official Full Name: ubiquitin thioesterase OTUB2
• NCBI Official Synonym Full Names: OTU deubiquitinase, ubiquitin aldehyde binding 2
• NCBI Official Symbol: OTUB2
• NCBI Official Synonym Symbols: OTB2; OTU2; C14orf137
• NCBI Protein Information: ubiquitin thioesterase OTUB2; OTU domain, ubiquitin aldehyde binding 2; OTU domain-containing ubiquitin aldehyde-binding protein 2; deubiquitinating enzyme OTUB2; otubain-2; ubiquitin-specific protease otubain 2; ubiquitin-specific-processing protease OTU
• UniProt Protein Name: Ubiquitin thioesterase OTUB2
• Protein Family: Ubiquitin thioesterase
• UniProt Gene Name: OTUB2
• UniProt Synonym Gene Names: C14orf137; OTB2; OTU2
• UniProt Entry Name: OTUB2_HUMAN

NCBI Description

This gene encodes one of several deubiquitylating enzymes. Ubiquitin modification of proteins is needed for their stability and function; to reverse the process, deubiquityling enzymes remove ubiquitin. This protein contains an OTU domain and binds Ubal (ubiquitin aldehyde); an active cysteine protease site is present in the OTU domain. [provided by RefSeq, Aug 2011]

Uniprot Description

OTUB2: Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains. Belongs to the peptidase C65 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.12; Hydrolase; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 14q32.12

Cellular Component: nucleus

Molecular Function: ubiquitin-specific protease activity

Biological Process: protein deubiquitination; proteolysis