NOLA1 blocking peptide

NOLA1 Blocking Peptide

Cat. Num. AAA9620417

• Gene Names: GAR1; NOLA1
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: NOLA1; NOLA1 Blocking Peptide; Gar1; GAR1_HUMAN; H/ACA ribonucleoprotein complex subunit 1; nucleolar protein family A member 1 (H/ACA small nucleolar RNPs); Nucleolar protein family A member 1; snoRNP protein GAR1; NOLA1 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for NOLA1 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for NOLA1 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 9506713
• NCBI GeneID: 54433
• NCBI Accession #: NP_061856.1
• NCBI GenBank Nucleotide #: NM_018983.3
• UniProt Accession #: Q9NY12; Q5MJQ2
• Molecular Weight: 22,348 Da
• NCBI Official Full Name: H/ACA ribonucleoprotein complex subunit 1
• NCBI Official Synonym Full Names: GAR1 ribonucleoprotein
• NCBI Official Symbol: GAR1
• NCBI Official Synonym Symbols: NOLA1
• NCBI Protein Information: H/ACA ribonucleoprotein complex subunit 1; snoRNP protein GAR1; GAR1 ribonucleoprotein homolog; nucleolar protein family A member 1; nucleolar protein family A, member 1 (H/ACA small nucleolar RNPs)
• UniProt Protein Name: H/ACA ribonucleoprotein complex subunit 1
• UniProt Gene Name: GAR1
• UniProt Synonym Gene Names: NOLA1
• UniProt Entry Name: GAR1_HUMAN

Uniprot Description

NOLA1: Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine (psi) residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. Belongs to the GAR1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; RNA-binding

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: Cajal body; nucleolus; nucleus

Molecular Function: cation channel activity

Biological Process: pseudouridine synthesis; cation transport; rRNA processing