Niemann Pick C1 Blocking Peptide | NPC1 blocking peptide

Niemann Pick C1 Peptide

Cat. Num. AAA544052

• Gene Names: NPC1; NPC
• Applications: Immunocompetition, Immunodepletion
• Synonyms: Niemann Pick C1; Niemann Pick C1 Peptide; Niemann Pick C1 protein precursor; Niemann Pick disease; type C1; NPC; NPC1 antibody; NPC1 blocking peptide

• Form/Format: Antigenic Blocking Peptide
• Concentration: Quantity: 250 ug; Volume: 100 ul (varies by lot)
• Sequence Length: 1278

• Applicable Applications for NPC1 blocking peptide: Immunocompetition, Immunodepletion
• Application Notes: Immunocytochemistry: 1:100; Immunofluorescence: 1:100; Immunohistochemistry: 1:100; Western Blot: 1:500
• Immunogen: Synthetic peptide taken within amino acid region 1228-1278 on human Niemann-Pick C1 protein.
• Expression: Adrenal gland; Cerebral cortex; lung; placenta; lymph node
• Molecular Function: Receptor activity
• Structure: Belongs to the patched family.
• Subcellular Location: Endosome, Lysosome, Membrane
• Preparation and Storage: -20 degree C for long term storage

Related Product Information for NPC1 blocking peptide

Intracellular cholesterol transporter plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment.

NCBI and Uniprot Product Information

• NCBI GI #: 255652944
• NCBI GeneID: 4864
• NCBI Accession #: NP_000262.2
• NCBI GenBank Nucleotide #: NM_000271.4
• UniProt Accession #: O15118; Q9P130; B4DET3
• Molecular Weight: 107,025 Da
• NCBI Official Full Name: Niemann-Pick C1 protein
• NCBI Official Synonym Full Names: NPC intracellular cholesterol transporter 1
• NCBI Official Symbol: NPC1
• NCBI Official Synonym Symbols: NPC
• NCBI Protein Information: Niemann-Pick C1 protein
• UniProt Protein Name: Niemann-Pick C1 protein
• Protein Family: Niemann-Pick C1 protein
• UniProt Gene Name: NPC1
• UniProt Entry Name: NPC1_HUMAN

NCBI Description

This gene encodes a large protein that resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. It is predicted to have a cytoplasmic C-terminus, 13 transmembrane domains, and 3 large loops in the lumen of the endosome - the last loop being at the N-terminus. This protein transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolized and released as free cholesterol. Defects in this gene cause Niemann-Pick type C disease, a rare autosomal recessive neurodegenerative disorder characterized by over accumulation of cholesterol and glycosphingolipids in late endosomal/lysosomal compartments.[provided by RefSeq, Aug 2009]

Uniprot Description

NPC1: Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals. Defects in NPC1 are the cause of Niemann-Pick disease type C1 (NPC1). A lysosomal storage disorder that affects the viscera and the central nervous system. It is due to defective intracellular processing and transport of low-density lipoprotein derived cholesterol. It causes accumulation of cholesterol in lysosomes, with delayed induction of cholesterol homeostatic reactions. Niemann-Pick disease type C1 has a highly variable clinical phenotype. Clinical features include variable hepatosplenomegaly and severe progressive neurological dysfunction such as ataxia, dystonia and dementia. The age of onset can vary from infancy to late adulthood. An allelic variant of Niemann-Pick disease type C1 is found in people with Nova Scotia ancestry. Patients with the Nova Scotian clinical variant are less severely affected. Belongs to the patched family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 18q11.2

Cellular Component: endoplasmic reticulum; extracellular region; Golgi apparatus; integral to membrane; integral to plasma membrane; late endosome membrane; lipid raft; lysosomal membrane; lysosome; membrane; nuclear envelope; perinuclear region of cytoplasm

Molecular Function: cholesterol binding; hedgehog receptor activity; protein binding; receptor activity; sterol transporter activity; transmembrane receptor activity; viral receptor activity

Biological Process: adult walking behavior; autophagy; bile acid metabolic process; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; endocytosis; entry of virus into host cell; lipid raft organization and biogenesis; lysosomal transport; negative regulation of macroautophagy; protein amino acid glycosylation; response to cadmium ion; response to drug; signal transduction

Disease: Niemann-pick Disease, Type C1