Myt1 blocking peptide

Myt1 Blocking Peptide

Cat. Num. AAA9618944

• Gene Names: PKMYT1; MYT1
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: Myt1; Myt1 Blocking Peptide; cdc2 inhibitory kinase; DKFZp547K1610; FLJ20093; Membrane associated tyrosine and threonine specific cdc2 inhibitory kinase; Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; MYT1; Myt1 kinase; PKMYT 1; Pkmyt1; PMYT1_HUMAN; PPP1R126; Protein kinase membrane associated tyrosine/threonine 1; Protein kinase Myt1; Protein phosphatase 1 regulatory subunit 126; Myt1 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for Myt1 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Myt1 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 33383241
• NCBI GeneID: 9088
• NCBI Accession #: NP_004194.3
• NCBI GenBank Nucleotide #: NP_004194.3
• UniProt Accession #: Q99640; O14731; Q7LE24; Q8TCM9; D3DUA4; F8W164
• Molecular Weight: 54,521 Da
• NCBI Official Full Name: membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase isoform 1
• NCBI Official Synonym Full Names: protein kinase, membrane associated tyrosine/threonine 1
• NCBI Official Symbol: PKMYT1
• NCBI Official Synonym Symbols: MYT1
• NCBI Protein Information: membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; myt1 kinase
• UniProt Protein Name: Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
• Protein Family: Myelin transcription factor
• UniProt Gene Name: PKMYT1
• UniProt Synonym Gene Names: MYT1
• UniProt Entry Name: PMYT1_HUMAN

NCBI Description

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Uniprot Description

Myt1: a dual specificity protein kinase of the WEE family. Preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Alternative splice-variant isoforms have been reported.

Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, Other; Protein kinase, dual-specificity (non-receptor); Other group; WEE family

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; kinase activity; ATP binding; protein kinase activity

Biological Process: mitosis; regulation of cell cycle; regulation of mitosis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; protein amino acid phosphorylation; G1/S transition of mitotic cell cycle