MYH3 blocking peptide

MYH3 Blocking Peptide

Cat. Num. AAA9628668

• Gene Names: MYH3; DA8; DA2A; DA2B; HEMHC; SMHCE; MYHSE1; MYHC-EMB
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: MYH3; MYH3 Blocking Peptide; adult 1; MYH1; MYH1_HUMAN; MYH2; MYH4; MYH6; MyHC-2x; MyHC-IIx/d; MYL1; MYL2; MYL3; MYL5; MYL6; Myosin light chain 2 regulatory cardiac slow; Myosin 1; Myosin heavy chain 1; Myosin heavy chain 1 skeletal muscle adult; Myosin heavy chain 2 skeletal muscle adult; Myosin heavy chain 2x; Myosin heavy chain 3 skeletal muscle adult; Myosin heavy chain 4 skeletal muscle adult; Myosin heavy chain 6 skeletal muscle adult; Myosin heavy chain; Myosin heavy chain IIx/d; Myosin heavy chain skeletal muscle adult 1; Myosin II; Myosin light chain 1 alkali skeletal fast; Myosin light chain 3 alkali ventricular skeletal slow; Myosin light chain 4 alkali atrial embryonic; Myosin light chain 5 regulatory; Myosin light chain 6 alkali smooth muscle and non muscle; Myosin-1; Myosin1; skeletal muscle; MYH3 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for MYH3 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for MYH3 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 98986453
• NCBI GeneID: 4621
• NCBI Accession #: NP_002461.2
• NCBI GenBank Nucleotide #: NM_002470.3
• UniProt Accession #: P11055; Q15492
• Molecular Weight: 223,905 Da
• NCBI Official Full Name: myosin-3
• NCBI Official Synonym Full Names: myosin heavy chain 3
• NCBI Official Symbol: MYH3
• NCBI Official Synonym Symbols: DA8; DA2A; DA2B; HEMHC; SMHCE; MYHSE1; MYHC-EMB
• NCBI Protein Information: myosin-3
• UniProt Protein Name: Myosin-3
• UniProt Gene Name: MYH3
• UniProt Entry Name: MYH3_HUMAN

NCBI Description

Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. This gene is a member of the MYH family and encodes a protein with an IQ domain and a myosin head-like domain. Mutations in this gene have been associated with two congenital contracture (arthrogryposis) syndromes, Freeman-Sheldon syndrome and Sheldon-Hall syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

MYH3: Muscle contraction. Defects in MYH3 are the cause of distal arthrogryposis type 2A (DA2A); also known as Freeman-Sheldon syndrome (FSS). Distal arthrogryposis is a clinically and genetically heterogeneous group of disorders characterized by bone anomalies and joint contractures of the hands and feet, causing medially overlapping fingers, clenched fists, ulnar deviation of fingers, camptodactyly and positional foot deformities. It is a disorder of primary limb malformation without primary neurologic or muscle disease. DA2A is the most severe form of distal arthrogryposis. Affected individuals have contractures of the orofacial muscles, characterized by microstomia with pouting lips, H-shaped dimpling of the chin, deep nasolabial folds, and blepharophimosis. Dysphagia, failure to thrive, growth deficit, and life-threatening respiratory complications (caused by structural anomalies of the oropharynx and upper airways) are frequent. Inheritance is autosomal dominant. Defects in MYH3 are the cause of distal arthrogryposis type 2B (DA2B); also known as Sheldon-Hall syndrome (SHS) or arthrogryposis multiplex congenita distal type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. DA2B is the most common of the distal arthrogryposis syndromes. It is similar to DA2A but the facial contractures are less dramatic.

Protein type: Motor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: sarcomere; muscle myosin complex; cytosol

Molecular Function: microfilament motor activity; calmodulin binding; actin filament binding; ATPase activity, coupled; ATP binding

Biological Process: skeletal muscle contraction; muscle development; actin filament-based movement; metabolic process; sarcomere organization; muscle filament sliding; embryonic limb morphogenesis

Disease: Arthrogryposis, Distal, Type 8; Arthrogryposis, Distal, Type 2a; Arthrogryposis, Distal, Type 2b