MBD3 blocking peptide

MBD3 Antibody (N-term) Blocking peptide

• Synonyms: MBD3; MBD3 Antibody (N-term) Blocking peptide; Methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3; MBD3 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of MBD3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 291

• Cellular Location: Nucleus. Chromosome. Note: Nuclear, in discrete foci. Detected on chromatin, at promoter regions of active genes
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for MBD3 blocking peptide

Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases.

NCBI and Uniprot Product Information

• NCBI GI #: 50400820
• NCBI GeneID: 53615
• UniProt Accession #: O95983; Q6PIL9; Q6PJZ9; Q86XF4; A8K4B7; D6W5Z2
• Molecular Weight: 29,014 Da
• NCBI Official Full Name: Methyl-CpG-binding domain protein 3
• NCBI Official Synonym Full Names: methyl-CpG binding domain protein 3
• NCBI Official Symbol: MBD3
• NCBI Protein Information: methyl-CpG-binding domain protein 3
• UniProt Protein Name: Methyl-CpG-binding domain protein 3
• Protein Family: Methyl-CpG-binding domain protein
• UniProt Gene Name: MBD3
• UniProt Entry Name: MBD3_HUMAN

NCBI Description

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). The encoded protein is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is not capable of binding to methylated DNA. The protein mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

MBD3: methyl-CpG binding (MBD) proteins appear to bind differentially methylated domains (DMDs) of DNA, to recruit repression complexes, and to silence the locus. The methyl CpG binding domain of human MBD3 may not directly bind mCpG but does interact with the NuRD/Mi2 components HDAC1 and MTA2. Plays a role in histone deacetylation and nucleosome remodeling. Increased levels have been reported in lung cancer and glioblastomas and may be a therapeutic target.

Protein type: Transcription, coactivator/corepressor; Gene silencing

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nuclear chromatin; nucleoplasm; protein complex

Molecular Function: DNA binding; histone deacetylase activity; methyl-CpG binding; nucleosomal DNA binding; protein binding

Biological Process: ATP-dependent chromatin remodeling; methylation-dependent chromatin silencing; negative regulation of transcription from RNA polymerase II promoter