LRWD1 blocking peptide

LRWD1 Antibody (N-term) Blocking peptide

• Gene Names: LRWD1; ORCA; CENP-33
• Synonyms: LRWD1; LRWD1 Antibody (N-term) Blocking peptide; Leucine-rich repeat and WD repeat-containing protein 1; Centromere protein 33; CENP-33; Origin recognition complex-associated protein; ORC-associated protein; ORCA; CENP33; LRWD1 blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 647

• Cellular Location: Nucleus. Chromosome, centromere. Chromosome, telomere. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note: Localizes to heterochromatin during G1 phase. Restricted to centromeres or telomeres as cells progress though S phase. When cells enter mitosis, relocalizes to centromeres. Recruitment to pericentric heterochromatin largely depends on the presence of H3K9me3
• Tissue Location: Testis-specific. Drastically down-regulated in testis from patients with Sertoli cell-only syndrome (SCOS)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for LRWD1 blocking peptide

Required for G1/S transition. Recruits and stabilizes the origin recognition complex (ORC) onto chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K20me3 and H4K27me3 in a cooperative manner with DNA methylation. Required for silencing of major satellite repeats. May be important ORC2, ORC3 and ORC4 stability.

NCBI and Uniprot Product Information

• NCBI GI #: 23097240
• NCBI GeneID: 222229
• NCBI Accession #: NP_690852.1
• NCBI GenBank Nucleotide #: NM_152892.2
• UniProt Accession #: Q9UFC0; Q8N0T9; Q8WV43; Q96GJ2; A8K4K2; B2R9G2
• Molecular Weight: 70,861 Da
• NCBI Official Full Name: leucine-rich repeat and WD repeat-containing protein 1 isoform 1
• NCBI Official Synonym Full Names: leucine rich repeats and WD repeat domain containing 1
• NCBI Official Symbol: LRWD1
• NCBI Official Synonym Symbols: ORCA; CENP-33
• NCBI Protein Information: leucine-rich repeat and WD repeat-containing protein 1
• UniProt Protein Name: Leucine-rich repeat and WD repeat-containing protein 1
• Protein Family: Leucine-rich repeat and WD repeat-containing protein
• UniProt Gene Name: LRWD1
• UniProt Synonym Gene Names: CENP-33; ORC-associated protein; ORCA
• UniProt Entry Name: LRWD1_HUMAN

NCBI Description

The protein encoded by this gene interacts with components of the origin recognition complex (ORC) and regulates the formation of the prereplicative complex. The encoded protein stabilizes the ORC and therefore aids in DNA replication. This protein is required for the G1/S phase transition of the cell cycle. In addition, the encoded protein binds to trimethylated histone H3 in heterochromatin and recruits the ORC and lysine methyltransferases, which help maintain the repressive heterochromatic state. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2015]

Uniprot Description

LRWD1: Associated component of the origin recognition complex (ORC) required to recruit and stabilize the ORC complex to chromatin. Probably required for the association of ORC on chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin: binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation. It is unclear whether it recongnizes and binds these repressive marks by itself or needs additional factors. Belongs to the LRWD1 family.

Protein type: DNA replication; Histone-binding; DNA-binding

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: centric heterochromatin; nuclear origin of replication recognition complex; nucleus

Molecular Function: chromatin binding; methyl-CpG binding; methylated histone residue binding; protein binding

Biological Process: DNA replication initiation; establishment and/or maintenance of chromatin architecture