JMJD6 blocking peptide

JMJD6 Antibody (C-term) Blocking peptide

• Gene Names: JMJD6; PSR; PTDSR; PTDSR1
• Synonyms: JMJD6; JMJD6 Antibody (C-term) Blocking peptide; Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6; 11411-; Histone arginine demethylase JMJD6; JmjC domain-containing protein 6; Jumonji domain-containing protein 6; Lysyl-hydroxylase JMJD6; Peptide-lysine 5-dioxygenase JMJD6; Phosphatidylserine receptor; Protein PTDSR; KIAA0585; PTDSR; JMJD6 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of JMJD6. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 403

• Cellular Location: Nucleus, nucleoplasm. Nucleus, nucleolus. Note: Mainly found throughout the nucleoplasm outside of regions containing heterochromatic DNA, with some localization in nucleolus. During mitosis, excluded from the nucleus and reappears in the telophase of the cell cycle
• Tissue Location: Highly expressed in the heart, skeletal muscle and kidney. Expressed at moderate or low level in brain, placenta, lung, liver, pancreas, spleen, thymus, prostate, testis and ovary Up-regulated in many patients with chronic pancreatitis. Expressed in nursing thymic epithelial cells.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for JMJD6 blocking peptide

Dioxygenase that can both act as a histone arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Acts as a regulator of RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as an RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. However, histone arginine demethylation may not constitute the primary activity in vivo. Has no histone lysine demethylase activity. Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65. Seems to be necessary for the regulation of macrophage cytokine responses.

NCBI and Uniprot Product Information

• NCBI GI #: 67461014
• NCBI GeneID: 23210
• UniProt Accession #: Q6NYC1; Q86VY0; Q8IUM5; Q9Y4E2; B3KMN8; B4DGX1
• Molecular Weight: 47,626 Da
• NCBI Official Full Name: Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6
• NCBI Official Synonym Full Names: arginine demethylase and lysine hydroxylase
• NCBI Official Symbol: JMJD6
• NCBI Official Synonym Symbols: PSR; PTDSR; PTDSR1
• NCBI Protein Information: bifunctional arginine demethylase and lysyl-hydroxylase JMJD6
• UniProt Protein Name: Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6
• Protein Family: Bifunctional arginine demethylase and lysyl-hydroxylase
• UniProt Gene Name: JMJD6
• UniProt Synonym Gene Names: KIAA0585; PTDSR; Protein PTDSR
• UniProt Entry Name: JMJD6_HUMAN

NCBI Description

This gene encodes a nuclear protein with a JmjC domain. JmjC domain-containing proteins are predicted to function as protein hydroxylases or histone demethylases. This protein was first identified as a putative phosphatidylserine receptor involved in phagocytosis of apoptotic cells; however, subsequent studies have indicated that it does not directly function in the clearance of apoptotic cells, and questioned whether it is a true phosphatidylserine receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

JMJD6: Dioxygenase that can both act as a histone arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Acts as a regulator of RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as a RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. However, histone arginine demethylation may not constitute the primary activity in vivo. Has no histone lysine demethylase activity. Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65. Seems to be necessary for the regulation of macrophage cytokine responses. Belongs to the JMJD6 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.11.-; Nucleolus; Oxidoreductase; Apoptosis; Cell development/differentiation; Receptor, misc.

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: nucleolus; nucleoplasm; nucleus

Molecular Function: histone demethylase activity; histone demethylase activity (H3-R2 specific); histone demethylase activity (H4-R3 specific); identical protein binding; iron ion binding; protein binding; RNA binding; single-stranded RNA binding

Biological Process: peptidyl-lysine hydroxylation to 5-hydroxy-L-lysine; regulation of nuclear mRNA splicing, via spliceosome; sprouting angiogenesis