INPP5E blocking peptide

INPP5E Blocking Peptide

Cat. Num. AAA9628160

• Gene Names: INPP5E; CPD4; CORS1; JBTS1; MORMS; PPI5PIV; pharbin
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: INPP5E; INPP5E Blocking Peptide; 5-bisphosphate 5-phosphatase; 72 kDa inositol polyphosphate 5-phosphatase; Inositol polyphosphate 5 phosphatase; INP5E_HUMAN; Inpp5e; Phosphatidylinositol (4; 5) bisphosphate 5 phosphatase; Phosphatidylinositol 4; Phosphatidylinositol polyphosphate 5 phosphatase type IV; Phosphatidylinositol polyphosphate 5-phosphatase type IV; PPI5PIV; INPP5E blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for INPP5E blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for INPP5E blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 972781524
• NCBI GeneID: 56623
• NCBI Accession #: NP_001305431.1
• NCBI GenBank Nucleotide #: NM_001318502.1
• UniProt Accession #: Q9NRR6; Q15734; Q6PIV5
• Molecular Weight: 66,193 Da
• NCBI Official Full Name: 72 kDa inositol polyphosphate 5-phosphatase isoform 2
• NCBI Official Synonym Full Names: inositol polyphosphate-5-phosphatase E
• NCBI Official Symbol: INPP5E
• NCBI Official Synonym Symbols: CPD4; CORS1; JBTS1; MORMS; PPI5PIV; pharbin
• NCBI Protein Information: 72 kDa inositol polyphosphate 5-phosphatase
• UniProt Protein Name: 72 kDa inositol polyphosphate 5-phosphatase
• Protein Family: 72 kDa inositol polyphosphate 5-phosphatase
• UniProt Gene Name: INPP5E
• UniProt Entry Name: INP5E_HUMAN

NCBI Description

The protein encoded by this gene is an inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase. InsP3 5-phosphatases hydrolyze Ins(1,4,5)P3, which mobilizes intracellular calcium and acts as a second messenger mediating cell responses to various stimulation. Studies of the mouse counterpart suggest that this protein may hydrolyze phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,5-bisphosphate on the cytoplasmic Golgi membrane and thereby regulate Golgi-vesicular trafficking. Mutations in this gene cause Joubert syndrome; a clinically and genetically heterogenous group of disorders characterized by midbrain-hindbrain malformation and various associated ciliopathies that include retinal dystrophy, nephronophthisis, liver fibrosis and polydactyly. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]

Uniprot Description

INPP5E: Converts phosphatidylinositol 3,4,5-trisphosphate (PtdIns 3,4,5-P3) to PtdIns-P2. Specific for lipid substrates, inactive towards water soluble inositol phosphates. Defects in INPP5E are the cause of Joubert syndrome type 1 (JBTS1). A disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay. Neuroradiologically, it is characterized by cerebellar vermian hypoplasia/aplasia, thickened and reoriented superior cerebellar peduncles, and an abnormally large interpeduncular fossa, giving the appearance of a molar tooth on transaxial slices (molar tooth sign). Additional variable features include retinal dystrophy and renal disease. Defects in INPP5E are the cause of mental retardation- truncal obesity-retinal dystrophy-micropenis (MORMS). An autosomal recessive disorder characterized by moderate mental retardation, truncal obesity, congenital non-progressive retinal dystrophy, and micropenis in males. The phenotype is similar to Bardet-Biedl syndrome and Cohen syndrome Distinguishing features are the age of onset, the non-progressive nature of the visual impairment, lack of dysmorphic facies, skin or gingival infection, microcephaly, mottled retina, polydactyly, and testicular anomalies. Belongs to the inositol 1,4,5-trisphosphate 5- phosphatase type IV family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Phosphatase (non-protein); Carbohydrate Metabolism - inositol phosphate; EC 3.1.3.36

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: ruffle; cytoskeleton; plasma membrane; axoneme; cytosol

Molecular Function: phosphoinositide 5-phosphatase activity; inositol-polyphosphate 5-phosphatase activity

Biological Process: phospholipid metabolic process; organelle organization and biogenesis; inositol phosphate dephosphorylation; phosphatidylinositol biosynthetic process; phosphoinositide dephosphorylation

Disease: Mental Retardation, Truncal Obesity, Retinal Dystrophy, And Micropenis Syndrome; Joubert Syndrome 1