HERC5 blocking peptide

HERC5 Blocking Peptide

Cat. Num. AAA9621762

• Gene Names: HERC5; CEB1; CEBP1
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: HERC5; HERC5 Blocking Peptide; CEB1; CEBP1; Cyclin E binding protein 1; Cyclin-E-binding protein 1; E3 ISG15--protein ligase HERC5; HECT domain and RCC1 like domain containing protein 5; HECT domain and RCC1-like domain-containing protein 5; Hect domain and RLD 5; HECT E3 ubiquitin ligase; HERC5_HUMAN; Herc6; HERC5 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for HERC5 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for HERC5 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 110825982
• NCBI GeneID: 51191
• NCBI Accession #: NP_057407
• NCBI GenBank Nucleotide #: NP_057407.2
• UniProt Accession #: Q9UII4; Q69G20; B2RTQ1
• Molecular Weight: 43 kDa (367aa)
• NCBI Official Full Name: E3 ISG15--protein ligase HERC5
• NCBI Official Synonym Full Names: HECT and RLD domain containing E3 ubiquitin protein ligase 5
• NCBI Official Symbol: HERC5
• NCBI Official Synonym Symbols: CEB1; CEBP1
• NCBI Protein Information: E3 ISG15--protein ligase HERC5
• UniProt Protein Name: E3 ISG15--protein ligase HERC5
• Protein Family: E3 ISG15--protein ligase
• UniProt Gene Name: HERC5
• UniProt Synonym Gene Names: CEB1; CEBP1

NCBI Description

This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4. [provided by RefSeq, Jul 2008]

Uniprot Description

Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Functions as part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets. Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant-negative effect on virus infectivity. Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner. In an interferon-stimulated cell, newly translated viral proteins are primary targets of ISG15.