GIRK1/KIR3.1/KCNJ3 blocking peptide

GIRK1/KIR3.1/KCNJ3 Blocking Peptide

Cat. Num. AAA9617763

• Gene Names: KCNJ3; KGA; GIRK1; KIR3.1
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: GIRK1/KIR3.1/KCNJ3; GIRK1/KIR3.1/KCNJ3 Blocking Peptide; G protein activated inward rectifier potassium channel 1; G protein-activated inward rectifier potassium channel 1; GIRK-1; GIRK1; Inward rectifier K (+) channel Kir3.1; Inwardly rectifying potassium channel KIR3.1; inwardly rectifying subfamily J member 3; IRK3; IRK3_HUMAN; Kcnf3; Kcnj3; KGA; KGB1; Kir3.1; Potassium channel; Potassium inwardly-rectifying channel; subfamily J; member 3; GIRK1/KIR3.1/KCNJ3 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for GIRK1/KIR3.1/KCNJ3 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for GIRK1/KIR3.1/KCNJ3 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 386781846
• NCBI GeneID: 3760
• NCBI Accession #: NP_001247437.1
• NCBI GenBank Nucleotide #: NM_001260508.1
• UniProt Accession #: P48549; Q8TBI0; B4DEW7
• Molecular Weight: 56,603 Da
• NCBI Official Full Name: G protein-activated inward rectifier potassium channel 1 isoform 2
• NCBI Official Synonym Full Names: potassium inwardly-rectifying channel, subfamily J, member 3
• NCBI Official Symbol: KCNJ3
• NCBI Official Synonym Symbols: KGA; GIRK1; KIR3.1
• NCBI Protein Information: G protein-activated inward rectifier potassium channel 1; GIRK-1; inward rectifier K+ channel KIR3.1; inward rectifier K(+) channel Kir3.1; potassium channel, inwardly rectifying subfamily J member 3; potassium inwardly-rectifying channel subfamily J memb
• UniProt Protein Name: G protein-activated inward rectifier potassium channel 1
• UniProt Gene Name: KCNJ3
• UniProt Synonym Gene Names: GIRK1; GIRK-1
• UniProt Entry Name: IRK3_HUMAN

NCBI Description

Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and plays an important role in regulating heartbeat. It associates with three other G-protein-activated potassium channels to form a heteromultimeric pore-forming complex that also couples to neurotransmitter receptors in the brain and whereby channel activation can inhibit action potential firing by hyperpolarizing the plasma membrane. These multimeric G-protein-gated inwardly-rectifying potassium (GIRK) channels may play a role in the pathophysiology of epilepsy, addiction, Down's syndrome, ataxia, and Parkinson's disease. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, May 2012]

Uniprot Description

GIRK1: This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This receptor plays a crucial role in regulating the heartbeat. Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ3 subfamily.

Protein type: Channel, potassium; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2q24.1

Cellular Component: voltage-gated potassium channel complex; T-tubule; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; G-protein activated inward rectifier potassium channel activity; inward rectifier potassium channel activity

Biological Process: synaptic transmission; potassium ion import; response to electrical stimulus; potassium ion transport