GALNT3 blocking peptide

GALNT3 Antibody (Center) Blocking Peptide

• Gene Names: GALNT3; HHS; HFTC; GalNAc-T3
• Synonyms: GALNT3; GALNT3 Antibody (Center) Blocking Peptide; Polypeptide N-acetylgalactosaminyltransferase 3; Polypeptide GalNAc transferase 3; GalNAc-T3; pp-GaNTase 3; Protein-UDP acetylgalactosaminyltransferase 3; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3; GALNT3 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the Center region of human GALNT3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 633

• Cellular Location: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note: Resides preferentially in the trans and medial parts of the Golgi stack
• Tissue Location: Expressed in organs that contain secretory epithelial glands. Highly expressed in pancreas, skin, kidney and testis. Weakly expressed in prostate, ovary, intestine and colon Also expressed in placenta and lung and fetal lung and fetal kidney.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for GALNT3 blocking peptide

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D- galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward HIV envelope glycoprotein gp120, EA2, Muc2 and Muc5. Probably glycosylates fibronectin in vivo. Glycosylates FGF23. Plays a central role in phosphate homeostasis.

NCBI and Uniprot Product Information

• NCBI GI #: 209572629
• NCBI GeneID: 2591
• UniProt Accession #: Q14435; Q53TG9; Q7Z476
• Molecular Weight: 22,212 Da
• NCBI Official Full Name: Polypeptide N-acetylgalactosaminyltransferase 3
• NCBI Official Synonym Full Names: polypeptide N-acetylgalactosaminyltransferase 3
• NCBI Official Symbol: GALNT3
• NCBI Official Synonym Symbols: HHS; HFTC; GalNAc-T3
• NCBI Protein Information: polypeptide N-acetylgalactosaminyltransferase 3
• UniProt Protein Name: Polypeptide N-acetylgalactosaminyltransferase 3
• UniProt Gene Name: GALNT3
• UniProt Synonym Gene Names: GalNAc-T3; pp-GaNTase 3
• UniProt Entry Name: GALT3_HUMAN

NCBI Description

This gene encodes UDP-GalNAc transferase 3, a member of the GalNAc-transferases family. This family transfers an N-acetyl galactosamine to the hydroxyl group of a serine or threonine residue in the first step of O-linked oligosaccharide biosynthesis. Individual GalNAc-transferases have distinct activities and initiation of O-glycosylation is regulated by a repertoire of GalNAc-transferases. The protein encoded by this gene is highly homologous to other family members, however the enzymes have different substrate specificities. [provided by RefSeq, Jul 2008]

Uniprot Description

GALNT3: an enzyme that catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. A single-pass type II membrane protein that localizes to the Golgi apparatus membrane. Has activity toward HIV envelope glycoprotein gp120, EA2, Muc2 and Muc5. Probably glycosylates fibronectin in vivo. Plays a central role in phosphate homeostasis. Mutations cause cause familial tumoral calcinosis, a severe autosomal recessive metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Overexpressed in many differentiated carcinomas, suggesting that it may serve as a marker of tumor differentiation. Two alternatively spliced human isoforms have been described.

Protein type: Transferase; Glycan Metabolism - O-glycan biosynthesis; Membrane protein, integral; EC 2.4.1.41

Chromosomal Location of Human Ortholog: 2q24-q31

Cellular Component: Golgi apparatus; Golgi membrane; membrane; perinuclear region of cytoplasm

Molecular Function: calcium ion binding; manganese ion binding; polypeptide N-acetylgalactosaminyltransferase activity

Biological Process: fibroblast growth factor receptor signaling pathway; O-glycan processing; protein amino acid O-linked glycosylation via serine; protein amino acid O-linked glycosylation via threonine

Disease: Tumoral Calcinosis, Hyperphosphatemic, Familial