GAK blocking peptide

GAK Antibody (C-term) Blocking Peptide

• Gene Names: GAK; DNAJ26; DNAJC26
• Synonyms: GAK; GAK Antibody (C-term) Blocking Peptide; Cyclin-G-associated kinase; GAK blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human GAK. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 1311

• Cellular Location: Cytoplasm, perinuclear region. Golgi apparatus, trans-Golgi network. Cell junction, focal adhesion. Note: Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions
• Tissue Location: Ubiquitous. Highest in testis.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for GAK blocking peptide

Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin- coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.

NCBI and Uniprot Product Information

• NCBI GI #: 17375734
• NCBI GeneID: 2580
• UniProt Accession #: O14976; Q5U4P5; Q9BVY6
• Molecular Weight: 134,464 Da
• NCBI Official Full Name: Cyclin-G-associated kinase
• NCBI Official Synonym Full Names: cyclin G associated kinase
• NCBI Official Symbol: GAK
• NCBI Official Synonym Symbols: DNAJ26; DNAJC26
• NCBI Protein Information: cyclin-G-associated kinase
• UniProt Protein Name: Cyclin-G-associated kinase
• Protein Family: Cyclin-G-associated kinase
• UniProt Gene Name: GAK
• UniProt Entry Name: GAK_HUMAN

NCBI Description

In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]

Uniprot Description

GAK: Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin- coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Protein type: Cell cycle regulation; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; EC 2.7.11.1; Kinase, protein; Other group; NAK family

Chromosomal Location of Human Ortholog: 4p16

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; membrane; vesicle

Molecular Function: protein binding

Biological Process: Golgi organization and biogenesis