Rabbit anti-Human COLEC11 Polyclonal Antibody | anti-COLEC11 antibody

COLEC11, NT (Collectin-11, Collectin Kidney Protein 1, CL-K1, UNQ596/PRO1182) (MaxLight 490)

Cat. Num. AAA6469936

• Gene Names: COLEC11; 3MC2; CLK1; CL-K1-I; CL-K1-II; CL-K1-IIa; CL-K1-IIb
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A/G Affinity Chromatography
• Synonyms: COLEC11; Polyclonal Antibody; NT (Collectin-11; Collectin Kidney Protein 1; CL-K1; UNQ596/PRO1182) (MaxLight 490); anti-COLEC11 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human COLEC11.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
• Sequence Length: 271

• Applicable Applications for anti-COLEC11 antibody: FLISA, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 50-78 in the N-terminal region of human COLEC11, UniProt Accession #Q9BWP8, NP_076932.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MDA-MB231 cell line lysates (35ug/lane) usingMBS623755. COLEC11 (arrow) was detected usingMBS623755.)

Western Blot (WB)

(Western Blot analysis of 293 cell lysates (2ug/lane) using MBS623755 Lane 1: nontransfected Lane 2: transiently transfected with the COLEC11 gene.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma usingMBS623755, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS623755 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MDA-231 cells using MBS623755 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-COLEC11 antibody

COLEC11 is a member of the collectin family of C-type lectins, which contain a collagen-like domain and a carbohydrate recognition domain, and play a role in host-defense

Product Categories/Family for anti-COLEC11 antibody

Antibodies; Binding Proteins

References

1. Oguri, M., et.al., Am. J. Hypertens. (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 13128972
• NCBI GeneID: 78989
• NCBI Accession #: NP_076932
• NCBI GenBank Nucleotide #: NP_076932.1
• UniProt Accession #: Q9BWP8
• Molecular Weight: 28.665
• NCBI Official Full Name: collectin-11 isoform a
• NCBI Official Synonym Full Names: collectin subfamily member 11
• NCBI Official Symbol: COLEC11
• NCBI Official Synonym Symbols: 3MC2; CLK1; CL-K1-I; CL-K1-II; CL-K1-IIa; CL-K1-IIb
• NCBI Protein Information: collectin-11
• UniProt Protein Name: Collectin-11
• Protein Family: Collectin
• UniProt Gene Name: COLEC11
• UniProt Synonym Gene Names: CL-K1

NCBI Description

This gene encodes a member of the collectin family of C-type lectins that possess collagen-like sequences and carbohydrate recognition domains. Collectins are secreted proteins that play important roles in the innate immune system by binding to carbohydrate antigens on microorganisms, facilitating their recognition and removal. The encoded protein binds to multiple sugars with a preference for fucose and mannose. Mutations in this gene are a cause of 3MC syndrome-2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

Lectin that plays a role in innate immunity, apoptosis and embryogenesis (PubMed:23954398, PubMed:25912189, PubMed:21258343). Calcium-dependent lectin that binds self and non-self glycoproteins presenting high mannose oligosaccharides with at least one terminal alpha-1,2-linked mannose epitope (PubMed:25912189). Primarily recognizes the terminal disaccharide of the glycan (PubMed:25912189). Also recognizes a subset of fucosylated glycans and lipopolysaccharides (PubMed:17179669, PubMed:25912189). Plays a role in innate immunity through its ability to bind non-self sugars presented by microorganisms and to activate the complement through the recruitment of MAPS1 (PubMed:20956340, PubMed:25912189). Also plays a role in apoptosis through its ability to bind in a calcium-independent manner the DNA present at the surface of apoptotic cells and to activate the complement in response to this binding (Probable). Finally, plays a role in development, probably serving as a guidance cue during the migration of neural crest cells and other cell types during embryogenesis (PubMed:21258343, PubMed:28301481).