FYN blocking peptide

FYN Antibody (N-term) Blocking Peptide

• Gene Names: FYN; SLK; SYN; p59-FYN
• Synonyms: FYN; FYN Antibody (N-term) Blocking Peptide; Tyrosine-protein kinase Fyn; Proto-oncogene Syn; Proto-oncogene c-Fyn; Src-like kinase; SLK; p59-Fyn; FYN blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human FYN. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 537

• Cellular Location: Cytoplasm. Nucleus. Cell membrane. Note: Present and active in lipid rafts. Palmitoylation is crucial for proper trafficking
• Tissue Location: Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T-lymphocytes.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for FYN blocking peptide

Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta- catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1.

NCBI and Uniprot Product Information

• NCBI GI #: 125370
• NCBI GeneID: 2534
• UniProt Accession #: P06241; Q16248; Q5R3A6; Q5R3A7; Q8N5D7; B5BU57; E1P557; H0UI48
• Molecular Weight: 54,513 Da
• NCBI Official Full Name: Tyrosine-protein kinase Fyn
• NCBI Official Synonym Full Names: FYN proto-oncogene, Src family tyrosine kinase
• NCBI Official Symbol: FYN
• NCBI Official Synonym Symbols: SLK; SYN; p59-FYN
• NCBI Protein Information: tyrosine-protein kinase Fyn
• UniProt Protein Name: Tyrosine-protein kinase Fyn
• Protein Family: FYN-binding protein
• UniProt Gene Name: FYN
• UniProt Synonym Gene Names: SLK
• UniProt Entry Name: FYN_HUMAN

NCBI Description

This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq, Jul 2008]

Uniprot Description

Fyn: a tyrosine kinase of the Src family. Implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Three alternatively spliced isoforms have been described. Isoform 2 shows a greater ability to mobilize cytoplasmic calcium than isoform 1. Induced expression aids in cellular transformation and xenograft metastasis. In squamous cell carcinoma, Fyn transduces signals from EGFR and Src and is required for cell migration and invasiveness. Activity linked to migration in a murine melanoma model. Appears to block late stage development of neuroblastoma. Mouse knockout deficient in kindling response, a model for human epilepsy.

Protein type: Kinase, protein; Protein kinase, TK; Oncoprotein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); TK group; Src family

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cytosol; endosome; extrinsic to internal side of plasma membrane; lipid raft; plasma membrane

Molecular Function: ephrin receptor binding; glycoprotein binding; non-membrane spanning protein tyrosine kinase activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: axon guidance; blood coagulation; cell differentiation; cell migration; central nervous system development; ephrin receptor signaling pathway; feeding behavior; innate immune response; learning; leukocyte migration; MAPKKK cascade; peptidyl-tyrosine phosphorylation; phosphoinositide-mediated signaling; platelet activation; regulation of apoptosis; regulation of cell proliferation; regulation of defense response to virus by virus; regulation of phosphoinositide 3-kinase cascade; stimulatory C-type lectin receptor signaling pathway; T cell activation; T cell costimulation; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway; vascular endothelial growth factor receptor signaling pathway