FGFR4 blocking peptide

FGFR4 Antibody Blocking Peptide

• Gene Names: FGFR4; TKF; JTK2; CD334
• Synonyms: FGFR4; FGFR4 Antibody Blocking Peptide; Fibroblast growth factor receptor 4; FGFR-4; CD334; JTK2; TKF; FGFR4 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the region of human FGFR4. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 802

• Cellular Location: Cell membrane; Single-pass type I membrane protein. Endosome. Endoplasmic reticulum. Note: Internalized from the cell membrane to recycling endosomes, and from there back to the cell membrane Isoform 3: Cytoplasm
• Tissue Location: Expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for FGFR4 blocking peptide

Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling.

NCBI and Uniprot Product Information

• NCBI GI #: 13432140
• NCBI GeneID: 2264
• UniProt Accession #: P22455; O43785; Q14309; Q71TW8; Q8TDA0; Q96KE5; G3JVM2; G3JVM5; G3JVM7; G3JVM9; AAB25788
• Molecular Weight: 64,640 Da
• NCBI Official Full Name: Fibroblast growth factor receptor 4
• NCBI Official Synonym Full Names: fibroblast growth factor receptor 4
• NCBI Official Symbol: FGFR4
• NCBI Official Synonym Symbols: TKF; JTK2; CD334
• NCBI Protein Information: fibroblast growth factor receptor 4
• UniProt Protein Name: Fibroblast growth factor receptor 4
• Protein Family: Fibroblast growth factor receptor
• UniProt Gene Name: FGFR4
• UniProt Synonym Gene Names: JTK2; TKF; FGFR-4
• UniProt Entry Name: FGFR4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. The genomic organization of this gene, compared to members 1-3, encompasses 18 exons rather than 19 or 20. Although alternative splicing has been observed, there is no evidence that the C-terminal half of the IgIII domain of this protein varies between three alternate forms, as indicated for members 1-3. This particular family member preferentially binds acidic fibroblast growth factor and, although its specific function is unknown, it is overexpressed in gynecological tumor samples, suggesting a role in breast and ovarian tumorigenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

FGFR4: a tyrosine-kinase receptor that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of the MAP kinase and AKT signaling pathways. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling. Two isoforms of the human protein are formed by alternative splicing. This description may include information from UniProtKB

Protein type: Membrane protein, integral; Protein kinase, TK; Kinase, protein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; FGFR family

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: cytoplasm; endoplasmic reticulum; Golgi apparatus; integral to plasma membrane; intercellular junction; nucleoplasm; plasma membrane; transport vesicle

Molecular Function: 1-phosphatidylinositol-3-kinase activity; fibroblast growth factor binding; fibroblast growth factor receptor activity; heparin binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: cell migration; fibroblast growth factor receptor signaling pathway; glucose homeostasis; MAPKKK cascade; peptidyl-tyrosine phosphorylation; phosphate ion homeostasis; phosphoinositide-mediated signaling; positive regulation of catalytic activity; positive regulation of cell proliferation; positive regulation of proteolysis; protein amino acid autophosphorylation; regulation of lipid metabolic process; regulation of phosphoinositide 3-kinase cascade