ERCC2 blocking peptide

ERCC2 Blocking Peptide (C-term)

• Gene Names: ERCC2; EM9; TTD; XPD; TTD1; COFS2; TFIIH
• Synonyms: ERCC2; ERCC2 Blocking Peptide (C-term); TFIIH basal transcription factor complex helicase XPD subunit; Basic transcription factor 2 80 kDa subunit; BTF2 p80; CXPD; DNA excision repair protein ERCC-2; DNA repair protein complementing XP-D cells; TFIIH basal transcription factor complex 80 kDa subunit; TFIIH 80 kDa subunit; TFIIH p80; Xeroderma pigmentosum group D-complementing protein; XPD; XPDC; ERCC2 blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 687-702 of HUMAN ERCC2
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 760

• Cellular Location: Nucleus. Cytoplasm, cytoskeleton, spindle
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ERCC2 blocking peptide

ATP-dependent 5'-3' DNA helicase, component of the core- TFIIH basal transcription factor. Involved in nucleotide excision repair (NER) of DNA by opening DNA around the damage, and in RNA transcription by RNA polymerase II by anchoring the CDK-activating kinase (CAK) complex, composed of CDK7, cyclin H and MAT1, to the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers.

NCBI and Uniprot Product Information

• NCBI GI #: 119540
• NCBI GeneID: 2068
• UniProt Accession #: P18074; Q2TB78; Q2YDY2; Q7KZU6; Q8N721
• Molecular Weight: 46,274 Da
• NCBI Official Full Name: TFIIH basal transcription factor complex helicase XPD subunit
• NCBI Official Synonym Full Names: ERCC excision repair 2, TFIIH core complex helicase subunit
• NCBI Official Symbol: ERCC2
• NCBI Official Synonym Symbols: EM9; TTD; XPD; TTD1; COFS2; TFIIH
• NCBI Protein Information: TFIIH basal transcription factor complex helicase XPD subunit
• UniProt Protein Name: TFIIH basal transcription factor complex helicase XPD subunit
• Protein Family: TFIIH basal transcription factor complex helicase
• UniProt Gene Name: ERCC2
• UniProt Synonym Gene Names: XPD; XPDC; BTF2 p80; TFIIH 80 kDa subunit; TFIIH p80
• UniProt Entry Name: ERCC2_HUMAN

NCBI Description

The nucleotide excision repair pathway is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

ERCC2: ATP-dependent 5'-3' DNA helicase, component of the core- TFIIH basal transcription factor. Involved in nucleotide excision repair (NER) of DNA by opening DNA around the damage, and in RNA transcription by RNA polymerase II by anchoring the CDK-activating kinase (CAK) complex, composed of CDK7, cyclin H and MAT1, to the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. Might also have a role in aging process and could play a causative role in the generation of skin cancers. Belongs to the helicase family. RAD3/XPD subfamily. One of the six subunits forming the core-TFIIH basal transcription factor which associates with the CAK complex composed of CDK7, CCNH/cyclin H and MNAT1 to form the TFIIH basal transcription factor. The interaction with GTF2H2 results in the stimulation of the 5'-->3' helicase activity. Interacts with Epstein-Barr virus EBNA2.

Protein type: Helicase; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: cyclin-dependent protein kinase activating kinase holoenzyme complex; cytoplasm; holo TFIIH complex; nucleoplasm; nucleus; spindle

Molecular Function: 5'-3' DNA helicase activity; DNA-dependent ATPase activity; protein binding; protein C-terminus binding; protein kinase activity; protein N-terminus binding; RNA polymerase subunit kinase activity

Biological Process: apoptosis; chromosome segregation; hair cell differentiation; mRNA capping; nucleotide-excision repair; nucleotide-excision repair, DNA duplex unwinding; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 3'-to lesion; nucleotide-excision repair, DNA incision, 5'-to lesion; nucleotide-excision repair, preincision complex assembly; nucleotide-excision repair, preincision complex stabilization; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; response to oxidative stress; RNA elongation from RNA polymerase I promoter; RNA elongation from RNA polymerase II promoter; termination of RNA polymerase I transcription; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase I promoter; transcription initiation from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair; UV protection

Disease: Cerebrooculofacioskeletal Syndrome 2; Trichothiodystrophy, Photosensitive; Xeroderma Pigmentosum, Complementation Group D