Human EPS8 (Epidermal growth factor receptor kinase substrate 8) ELISA Kit | EPS8 elisa kit

Human EPS8 (Epidermal growth factor receptor kinase substrate 8) ELISA Kit

Cat. Num. AAA7606222

• Gene Names: EPS8; DFNB102
• Reactivity: Human
• Synonyms: EPS8 (Epidermal growth factor receptor kinase substrate 8); Human EPS8 (Epidermal growth factor receptor kinase substrate 8) ELISA Kit; Epidermal growth factor receptor kinase substrate 8; EPS8 elisa kit

• Reactivity: Human
• Sequence Length: 822

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EPS8 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- EPS8 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-EPS8 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the EPS8 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of EPS8 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 56682953
• NCBI GeneID: 2059
• NCBI Accession #: NP_004438.3
• NCBI GenBank Nucleotide #: NM_004447.5
• UniProt Accession #: Q12929; Q8N6J0; A6NMC3; A8K6W2; A8KA66; B4DX66
• Molecular Weight: 62,871 Da
• NCBI Official Full Name: epidermal growth factor receptor kinase substrate 8
• NCBI Official Synonym Full Names: epidermal growth factor receptor pathway substrate 8
• NCBI Official Symbol: EPS8
• NCBI Official Synonym Symbols: DFNB102
• NCBI Protein Information: epidermal growth factor receptor kinase substrate 8
• UniProt Protein Name: Epidermal growth factor receptor kinase substrate 8
• Protein Family: Epidermal growth factor receptor kinase substrate
• UniProt Gene Name: EPS8

NCBI Description

This gene encodes a member of the EPS8 family. This protein contains one PH domain and one SH3 domain. It functions as part of the EGFR pathway, though its exact role has not been determined. Highly similar proteins in other organisms are involved in the transduction of signals from Ras to Rac and growth factor-mediated actin remodeling. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

EPS8: an adaptor protein that is an epidermal growth factor receptor (EGFR) substrate. Enhances EGF-dependent mitogenic signals. Member of a family of actin barbing and capping proteins. Promotes filopodial protrusions. Complexes containing Eps8/Abi1/PI3K/Sos-1 mediate Rac activation following EGFR signaling. Involved in endocytosis of EGFR by binding to the Rab5-specific GTPase activating protein USP6N. Binds to IRSp53, which binds small the small GTPases Rac and Cdc42, and which regulates actin dynamics. Involved in the actin-remodeling activity in cultured cerebellar neurons. Regulates the actin-remodeling activities of NMDA and ethanol in neurons. Eps8 knockout mice show behavioral resistance to ethanol. Expressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 12p12.3

Cellular Component: cell cortex; stereocilium; vesicle

Molecular Function: actin binding; protein binding; Rac GTPase binding; SH3/SH2 adaptor activity

Biological Process: actin crosslink formation; actin filament bundle formation; barbed-end actin filament capping; cell proliferation; epidermal growth factor receptor signaling pathway; exit from mitosis; Rac protein signal transduction; regulation of actin filament length; regulation of cell shape; signal transduction

Disease: Deafness, Autosomal Recessive 102