EHD2 blocking peptide

EHD2 Blocking Peptide

Cat. Num. AAA9621676

• Gene Names: EHD2; PAST2
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: EHD2; EHD2 Blocking Peptide; EH domain containing 3; EHD3; PAST3; EHD2 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for EHD2 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for EHD2 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 21361462
• NCBI GeneID: 30846
• NCBI Accession #: NP_055416.2
• NCBI GenBank Nucleotide #: NP_055416.2
• UniProt Accession #: Q9NZN3; Q96CB6; B2RDH9; B4DNU6; Q9NZN4
• Molecular Weight: 46,059 Da
• NCBI Official Full Name: EH domain-containing protein 2
• NCBI Official Synonym Full Names: EH-domain containing 2
• NCBI Official Symbol: EHD2
• NCBI Official Synonym Symbols: PAST2
• NCBI Protein Information: EH domain-containing protein 2; PAST homolog 2; EH domain containing 2
• UniProt Protein Name: EH domain-containing protein 2
• Protein Family: EH domain-containing protein
• UniProt Gene Name: EHD2
• UniProt Entry Name: EHD2_HUMAN

NCBI Description

This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway. [provided by RefSeq, Jul 2008]

Uniprot Description

EHD2: Plays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Binds ATP; does not bind GTP.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: caveola; cytosol; endosome membrane; extrinsic to membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; recycling endosome membrane

Molecular Function: ATP binding; calcium ion binding; GTP binding; hydrolase activity; nucleic acid binding; protein binding; protein domain specific binding

Biological Process: blood coagulation; cortical actin cytoskeleton organization and biogenesis; endocytic recycling; endocytosis; metabolic process