EBI2 blocking peptide

EBI2 Blocking Peptide

Cat. Num. AAA9623007

• Gene Names: GPR183; EBI2
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: EBI2; EBI2 Blocking Peptide; ebi 2; EBV induced G protein coupled receptor 2; EBV-induced G-protein coupled receptor 2; ebv-induced gene 2; Epstein Barr virus induced gene 2 (lymphocyte specific G protein coupled receptor); Epstein Barr virus induced gene 2; epstein-barr virus induced gene 2; Epstein-Barr virus-induced G-protein coupled receptor 2; G protein-coupled receptor 183; G-protein coupled receptor 183; GP183_HUMAN; gpr183; hEBI2; Lymphocyte specific G protein coupled receptor; EBI2 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for EBI2 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for EBI2 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 4826706
• NCBI GeneID: 1880
• NCBI Accession #: NP_004942.1
• NCBI GenBank Nucleotide #: NM_004951.4
• UniProt Accession #: P32249; Q53F99; Q5JUH7; B2R8N5
• Molecular Weight: 41,224 Da
• NCBI Official Full Name: G-protein coupled receptor 183
• NCBI Official Synonym Full Names: G protein-coupled receptor 183
• NCBI Official Symbol: GPR183
• NCBI Official Synonym Symbols: EBI2
• NCBI Protein Information: G-protein coupled receptor 183; EBV-induced G-protein coupled receptor 2; epstein-Barr virus-induced G-protein coupled receptor 2; Epstein-Barr virus induced gene 2 (lymphocyte-specific G protein-coupled receptor)
• UniProt Protein Name: G-protein coupled receptor 183
• UniProt Gene Name: GPR183
• UniProt Synonym Gene Names: EBI2; EBI2; EBV-induced G-protein coupled receptor 2
• UniProt Entry Name: GP183_HUMAN

NCBI Description

This gene was identified by the up-regulation of its expression upon Epstein-Barr virus infection of primary B lymphocytes. This gene is predicted to encode a G protein-coupled receptor that is most closely related to the thrombin receptor. Expression of this gene was detected in B-lymphocyte cell lines and lymphoid tissues but not in T-lymphocyte cell lines or peripheral blood T lymphocytes. The function of this gene is unknown. [provided by RefSeq, Jul 2008]

Uniprot Description

GPR183: Receptor for oxysterol 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) and other related oxysterols. Binding of 7- alpha,25-OHC mediates the correct localization of B-cells during humoral immune responses. Promotes activated B-cell localization in the outer follicle and interfollicular regions. Its specific expression during B-cell maturation helps position B-cells appropriately for mounting T-dependent antibody responses. Signals constitutively through G(i)-alpha, but not throughG(s)-alpha or G(q)-alpha. Signals constitutively also via MAPK1/3 (ERK1/2). Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q32.3

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; oxysterol binding

Biological Process: mature B cell differentiation during immune response; G-protein coupled receptor protein signaling pathway; positive regulation of B cell proliferation; immune response; humoral immune response