DNAJB4 blocking peptide

DNAJB4 Blocking Peptide

Cat. Num. AAA9623767

• Gene Names: DNAJB4; DjB4; HLJ1; DNAJW
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: DNAJB4; DNAJB4 Blocking Peptide; DjB4; DnaJ (Hsp40) homolog subfamily B member 4; DnaJ homolog subfamily B member 4; DnaJ like heat shock protein 40; DNAJB 4; DNAJW; DNJB4_HUMAN; Heat shock 40 kDa protein 1 homolog; Heat shock protein 40 homolog; HLJ 1; HLJ1; HSP40 homolog; Human liver DnaJ-like protein; DNAJB4 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for DNAJB4 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for DNAJB4 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 952977816
• NCBI GeneID: 11080
• NCBI Accession #: NP_001304028.1
• NCBI GenBank Nucleotide #: NM_001317099.1
• UniProt Accession #: Q9UDY4; Q13431; B2R824
• Molecular Weight: 39kDa
• NCBI Official Full Name: dnaJ homolog subfamily B member 4 isoform a
• NCBI Official Synonym Full Names: DnaJ heat shock protein family (Hsp40) member B4
• NCBI Official Symbol: DNAJB4
• NCBI Official Synonym Symbols: DjB4; HLJ1; DNAJW
• NCBI Protein Information: dnaJ homolog subfamily B member 4
• UniProt Protein Name: DnaJ homolog subfamily B member 4
• Protein Family: DnaJ homolog subfamily
• UniProt Gene Name: DNAJB4
• UniProt Synonym Gene Names: DNAJW; HLJ1; HSP40 homolog
• UniProt Entry Name: DNJB4_HUMAN

NCBI Description

The protein encoded by this gene is a molecular chaperone, tumor suppressor, and member of the heat shock protein-40 family. The encoded protein binds the cell adhesion protein E-cadherin and targets it to the plasma membrane. This protein also binds incorrectly folded E-cadherin and targets it for endoplasmic reticulum-associated degradation. This gene is a strong tumor suppressor for colorectal carcinoma, and downregulation of it may serve as a good biomarker for predicting patient outcomes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

Uniprot Description

DNAJB4: Probable chaperone. Homodimer. The C-terminal section interacts with the C- terminal tail of OPRM1. Interacts also with SDIM1. By heat shock. Expressed in heart, pancreas and skeletal muscle, and to a lesser extent in brain, placenta and liver.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 1p31.1

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; chaperone binding; unfolded protein binding

Biological Process: protein folding; response to heat; response to unfolded protein