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Western Blot (WB) (Gel: 12%SDS-PAGE Lysate: 40 μ g Lane 1-6: 293T£¬Hela£¬PC-3£¬Lncap and 231 cell£¬Human fetal muscle tissue Primary antibody: 1/500 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 second)

Rabbit anti-Human NDUFB6 Polyclonal Antibody | anti-NDUFB6 antibody

NDUFB6 Antibody

Gene Names
NDUFB6; CI; B17
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purification
Synonyms
NDUFB6; Polyclonal Antibody; NDUFB6 Antibody; CI; B17; anti-NDUFB6 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total NDUFB6 protein.
Purity/Purification
Antigen Affinity Purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
128
Applicable Applications for anti-NDUFB6 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:10-1:50
Immunogen
Full length fusion protein of human NDUFB6
Immunogen Type
Protein
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Gel: 12%SDS-PAGE Lysate: 40 μ g Lane 1-6: 293T£¬Hela£¬PC-3£¬Lncap and 231 cell£¬Human fetal muscle tissue Primary antibody: 1/500 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 second)

Western Blot (WB) (Gel: 12%SDS-PAGE Lysate: 40 μ g Lane 1-6: 293T£¬Hela£¬PC-3£¬Lncap and 231 cell£¬Human fetal muscle tissue Primary antibody: 1/500 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 second)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using at dilution 1/20.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using at dilution 1/20.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using at dilution 1/20.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using at dilution 1/20.)
Related Product Information for anti-NDUFB6 antibody
The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity.It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoformshave been identified.
Product Categories/Family for anti-NDUFB6 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13,716 Da
NCBI Official Full Name
NADH dehydrogenase
NCBI Official Synonym Full Names
NADH:ubiquinone oxidoreductase subunit B6
NCBI Official Symbol
NDUFB6
NCBI Official Synonym Symbols
CI; B17
NCBI Protein Information
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6
UniProt Protein Name
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6
Protein Family
UniProt Gene Name
NDUFB6
UniProt Synonym Gene Names
CI-B17
UniProt Entry Name
NDUB6_HUMAN

Uniprot Description

NDUFB6: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFB6 subunit family.

Protein type: EC 1.6.5.3; EC 1.6.99.3; Energy Metabolism - oxidative phosphorylation; Membrane protein, integral; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: 9p21.1

Cellular Component: mitochondrial inner membrane; mitochondrial membrane; mitochondrial respiratory chain complex I; mitochondrion; nucleoplasm

Biological Process: mitochondrial electron transport, NADH to ubiquinone; mitochondrial respiratory chain complex I assembly

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Product Notes

The NDUFB6 ndufb6 (Catalog #AAA9415629) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NDUFB6 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NDUFB6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:500-1:2000 Immunohistochemistry: 1:10-1:50. Researchers should empirically determine the suitability of the NDUFB6 ndufb6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDUFB6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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