GZMB recombinant protein

GZMB, 21-247aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA206060

• Gene Names: GZMB; C11; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: GZMB; 21-247aa; Human; His tag; E Coli (Denatured); Granzyme B; CCPI; CGL-1; CGL1; CSP-B; CSPB; CTLA1; CTSGL1; HLP; SECT; GZMB recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris 8.0 containing 10% glycerol
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MIIGGHEAKP HSRPYMAYLM IWDQKSLKRC GGFLIQDDFV LTAAHCWGSS INVTLGAHNI KEQEPTQQFI PVKRPIPHPA YNPKNFSNDI MLLQLERKAK RTRAVQPLRL PSNKAQVKPG QTCSVAGWGQ TAPLGKHSHT LQEVKMTVQE DRKCESDLRH YYDSTIELCV GDPEIKKTSF KGDSGGPLVC NKVAQGIVSY GRNNGMPPRA CTKVSSFVHW IKKTMKRY
• Sequence Length: 247

• Applicable Applications for GZMB recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for GZMB recombinant protein

GZMB also known as granzyme B is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface nonself antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Recombinant human GZMB, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for GZMB recombinant protein

Apoptosis

References

Xu W., et al. (2014) Eur. J. Immunol. 44 (1), 275-284; Fritsch K., et al. (2013) Ann. Hematol. 92 (12), 1603-1609

NCBI and Uniprot Product Information

• NCBI GI #: 221625528
• NCBI GeneID: 3002
• NCBI Accession #: NP_004122
• NCBI GenBank Nucleotide #: NM_004131.5
• UniProt Accession #: P10144; Q8N1D2; Q9UCC1
• Molecular Weight: 27.8kDa (248aa)
• NCBI Official Full Name: granzyme B isoform 1 preproprotein
• NCBI Official Synonym Full Names: granzyme B
• NCBI Official Symbol: GZMB
• NCBI Official Synonym Symbols: C11; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
• NCBI Protein Information: granzyme B
• UniProt Protein Name: Granzyme B
• Protein Family: Granzyme
• UniProt Gene Name: GZMB
• UniProt Synonym Gene Names: CGL1; CSPB; CTLA1; GRB; CTSGL1; Lymphocyte protease; HLP

NCBI Description

This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016]

Uniprot Description

This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.