DCK blocking peptide

DCK Blocking Peptide

Cat. Num. AAA9625992

• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: DCK; DCK Blocking Peptide; dCK; DCK protein; DCK_HUMAN; Deoxycytidine kinase; Deoxynucleoside kinase; EC 2.7.1; EC 2.7.1.74; MGC117410; MGC138632; OTTHUMP00000219118; OTTHUMP00000219119; DCK blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for DCK blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for DCK blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 4503269
• NCBI GeneID: 1633
• NCBI Accession #: NP_000779.1
• NCBI GenBank Nucleotide #: NM_000788.2
• UniProt Accession #: P27707; Q5TZY7; Q6FI11; B2R8V6
• Molecular Weight: 30,519 Da
• NCBI Official Full Name: Homo sapiens deoxycytidine kinase (DCK), mRNA
• NCBI Official Synonym Full Names: deoxycytidine kinase
• NCBI Official Symbol: DCK
• NCBI Protein Information: deoxycytidine kinase; deoxynucleoside kinase
• UniProt Protein Name: Deoxycytidine kinase
• Protein Family: Deoxycytidine kinase
• UniProt Gene Name: DCK
• UniProt Synonym Gene Names: dCK
• UniProt Entry Name: DCK_HUMAN

NCBI Description

Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq, Jul 2008]

Uniprot Description

DCK: Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents. Belongs to the DCK/DGK family.

Protein type: Kinase, other; Nucleotide Metabolism - purine; EC 2.7.1.74; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 4q13.3-q21.1

Cellular Component: cytosol; nucleus

Molecular Function: protein homodimerization activity; nucleoside kinase activity; deoxycytidine kinase activity; drug binding; ATP binding

Biological Process: pyrimidine base metabolic process; pyrimidine nucleotide metabolic process; nucleotide biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside salvage; phosphorylation; purine salvage; purine base metabolic process; deoxyribonucleoside monophosphate biosynthetic process