DAB2 blocking peptide

DAB2 Antibody (C-term) Blocking Peptide

• Gene Names: DAB2; DOC2; DOC-2
• Synonyms: DAB2; DAB2 Antibody (C-term) Blocking Peptide; Disabled homolog 2; DOC-2; Differentially-expressed protein 2; DOC2; DAB2 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of humanB2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 770

• Cellular Location: Cytoplasm. Cytoplasmic vesicle, clathrin- coated vesicle membrane. Membrane, clathrin-coated pit Note: Colocalizes with large insert-containing isoforms of MYO6 at clathrin-coated pits/vesicles. During mitosis is progressively displaced from the membrane and translocated to the cytoplasm
• Tissue Location: Expressed in deep invaginations, inclusion cysts and the surface epithelial cells of the ovary. Also expressed in breast epithelial cells, spleen, thymus, prostate, testis, macrophages, fibroblasts, lung epithelial cells, placenta, brain stem, heart and small intestine. Expressed in kidney proximal tubular epithelial cells (at protein level)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for DAB2 blocking peptide

Adapter protein that functions as clathrin-associated sorting protein (CLASP) required for clathrin-mediated endocytosis of selected cargo proteins. Can bind and assemble clathrin, and binds simultaneously to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and cargos containg non-phosphorylated NPXY internalization motifs, such as the LDL receptor, to recruit them to clathrin-coated pits. Can function in clathrin-mediated endocytosis independently of the AP-2 complex. Involved in endocytosis of integrin beta-1; this function seems to redundant with the AP-2 complex and seems to require DAB2 binding to endocytosis accessory EH domain-containing proteins such as EPS15, EPS15L1 and ITSN1. Involved in endocytosis of cystic fibrosis transmembrane conductance regulator/CFTR. Involved in endocytosis of megalin/LRP2 lipoprotein receptor during embryonal development. Required for recycling of the TGF-beta receptor. Involved in CFTR trafficking to the late endosome. Involved in several receptor- mediated signaling pathways. Involved in TGF-beta receptor signaling and facilitates phosphorylation of the signal transducer SMAD2. Mediates TFG-beta-stimulated JNK activation. May inhibit the canoniocal Wnt/beta-catenin signaling pathway by stabilizing the beta-catenin destruction complex through a competing association with axin preventing its dephosphorylation through protein phosphatase 1 (PP1). Sequesters LRP6 towards clathrin- mediated endocytosis, leading to inhibition of Wnt/beta-catenin signaling. May activate non-canonical Wnt signaling. In cell surface growth factor/Ras signaling pathways proposed to inhibit ERK activation by interrupting the binding of GRB2 to SOS1 and to inhibit SRC by preventing its activating phosphorylation at 'Tyr- 419'. Proposed to be involved in modulation of androgen receptor (AR) signaling mediated by SRC activation; seems to compete with AR for interaction with SRC. Plays a role in the CSF-1 signal transduction pathway. Plays a role in cellular differentiation. Involved in cell positioning and formation of visceral endoderm (VE) during embryogenesis and proposed to be required in the VE to respond to Nodal signaling coming from the epiblast. Required for the epithelial to mesenchymal transition, a process necessary for proper embryonic development. May be involved in myeloid cell differentiation and can induce macrophage adhesion and spreading. May act as a tumor suppressor.

NCBI and Uniprot Product Information

• NCBI GI #: 145559465
• NCBI GeneID: 1601
• UniProt Accession #: P98082; Q13598; Q9BTY0; Q9UK04; A6NES5
• Molecular Weight: 80,228 Da
• NCBI Official Full Name: Disabled homolog 2
• NCBI Official Synonym Full Names: DAB2, clathrin adaptor protein
• NCBI Official Symbol: DAB2
• NCBI Official Synonym Symbols: DOC2; DOC-2
• NCBI Protein Information: disabled homolog 2
• UniProt Protein Name: Disabled homolog 2
• Protein Family: Disabled
• UniProt Gene Name: DAB2
• UniProt Synonym Gene Names: DOC2; DOC-2
• UniProt Entry Name: DAB2_HUMAN

NCBI Description

This gene encodes a mitogen-responsive phosphoprotein. It is expressed in normal ovarian epithelial cells, but is down-regulated or absent from ovarian carcinoma cell lines, suggesting its role as a tumor suppressor. This protein binds to the SH3 domains of GRB2, an adaptor protein that couples tyrosine kinase receptors to SOS (a guanine nucleotide exchange factor for Ras), via its C-terminal proline-rich sequences, and may thus modulate growth factor/Ras pathways by competing with SOS for binding to GRB2. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

DAB2: an adaptor protein that interacts with Grb2, myosin VI, SMAD2/3, DIP1/2, Dvl-3, the integrin betasubunit, and c-Src. Negatively regulates canonical Wnt signaling by stabilizing the beta-catenin degradation complex. A component of the CSF-1 signal transduction pathway. Phosphorylated DAB2 inhibits adhesion and integrin signaling. Two alternatively spliced isoforms are described.

Protein type: Adaptor/scaffold; Tumor suppressor

Chromosomal Location of Human Ortholog: 5p13.1

Cellular Component: clathrin-coated vesicle; coated pit; focal adhesion; intracellular membrane-bound organelle; lysosomal membrane; nucleolus; plasma membrane

Molecular Function: protein binding; protein C-terminus binding; SMAD binding

Biological Process: cell proliferation; integrin-mediated signaling pathway; leading edge cell differentiation; negative regulation of apoptosis; negative regulation of protein binding; negative regulation of transcription, DNA-dependent; positive regulation of cell migration; positive regulation of endocytosis; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein amino acid phosphorylation; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor beta receptor signaling pathway