CTTN blocking peptide

CTTN Antibody (Center) Blocking peptide

• Gene Names: CTTN; EMS1
• Synonyms: CTTN; CTTN Antibody (Center) Blocking peptide; Src substrate cortactin; Amplaxin; Oncogene EMS1; EMS1; CTTN blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the Center region of CTTN. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 550

• Cellular Location: Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle. Cell projection, dendrite. Cell projection. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, podosome. Cell junction. Cell junction, focal adhesion. Membrane, clathrin-coated pit. Cell projection, dendritic spine. Cytoplasm, cell cortex. Note: Colocalizes transiently with PTK2/FAK1 at focal adhesions (By similarity). Associated with membrane ruffles and lamellipodia. In the presence of CTTNBP2NL, colocalizes with stress fibers (By similarity). In the presence of CTTNBP2, localizes at the cell cortex (By similarity). In response to neuronal activation by glutamate, redistributes from dendritic spines to the dendritic shaft (By similarity). Colocalizes with DNM2 at the basis of filopodia in hippocampus neuron growth zones (By similarity).
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for CTTN blocking peptide

Contributes to the organization of the actin cytoskeleton and cell shape (PubMed:21296879). Plays a role in the formation of lamellipodia and in cell migration. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density (By similarity). Plays a role in the invasiveness of cancer cells, and the formation of metastases (PubMed:16636290). Plays a role in focal adhesion assembly and turnover (By similarity). In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement (PubMed:20861316). Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane (PubMed:17959782). Plays a role in receptor-mediated endocytosis via clathrin-coated pits (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 215273892
• NCBI GeneID: 2017
• UniProt Accession #: Q14247; Q8N707; Q96H99; AAB26248
• Molecular Weight: 57,467 Da
• NCBI Official Full Name: Src substrate cortactin
• NCBI Official Synonym Full Names: cortactin
• NCBI Official Symbol: CTTN
• NCBI Official Synonym Symbols: EMS1
• NCBI Protein Information: src substrate cortactin
• UniProt Protein Name: Src substrate cortactin
• Protein Family: CTTNBP2 N-terminal-like protein
• UniProt Gene Name: CTTN
• UniProt Synonym Gene Names: EMS1
• UniProt Entry Name: SRC8_HUMAN

NCBI Description

This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq, May 2010]

Uniprot Description

Cortactin: a cytoskeletal protein that that is involved in coordinating actin reorganization during cell movement. Localizes at the leading edge of lamellipodia during cell migration. Its amino-terminal acidic domain associates with the Arp2/3 and WASP complex at F-actin branches. The central region of the protein contains six repeats of 37 amino acids that are important in F-actin binding and cross-linking. The carboxy-terminus contains a proline-rich region and an SH3 domain that can interact with numerous scaffolding proteins, such as CortBP1 and Shank3. Cortactin may play a role in the organization of transmembrane receptors at postsynaptic densities (PSD) and tight junctions by linking scaffolding proteins to the actin network. It is degraded in a caspase-dependent manner during apoptosis. It is overexpressed in numerous cancers with poor patient prognosis. It may contribute to tumor cell invasion and metastasis. Two splice variant isoforms have been identified.

Protein type: Actin-binding; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: actin filament; cell cortex; coated pit; cortical cytoskeleton; cytoplasm; cytoskeleton; focal adhesion; Golgi apparatus; growth cone; intracellular membrane-bound organelle; lamellipodium; plasma membrane; ruffle; voltage-gated potassium channel complex

Molecular Function: profilin binding; protein binding

Biological Process: actin cytoskeleton reorganization; cell motility involved in cell locomotion; focal adhesion formation; intracellular protein transport; neurite morphogenesis; positive regulation of actin filament polymerization; positive regulation of smooth muscle contraction; receptor-mediated endocytosis; regulation of axon extension