CSK blocking peptide

CSK Antibody (N-term) Blocking peptide

• Synonyms: CSK; CSK Antibody (N-term) Blocking peptide; Tyrosine-protein kinase CSK; C-Src kinase; Protein-tyrosine kinase CYL; CSK blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of CSK. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 450

• Cellular Location: Cytoplasm. Cell membrane. Note: Mainly cytoplasmic, also present in lipid rafts.
• Tissue Location: Expressed in lung and macrophages.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for CSK blocking peptide

Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T- cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.

NCBI and Uniprot Product Information

• NCBI GI #: 729887
• NCBI GeneID: 1445
• UniProt Accession #: P41240; Q2M3N2; Q6FGZ6
• Molecular Weight: 50,704 Da
• NCBI Official Full Name: Tyrosine-protein kinase CSK
• NCBI Official Synonym Full Names: c-src tyrosine kinase
• NCBI Official Symbol: CSK
• NCBI Protein Information: tyrosine-protein kinase CSK
• UniProt Protein Name: Tyrosine-protein kinase CSK
• Protein Family: Chloroplast sensor kinase
• UniProt Gene Name: CSK
• UniProt Entry Name: CSK_HUMAN

Uniprot Description

CSK: a tyrosine kinase of the Csk family that phosphorylates and inhibits Src family kinases. Specifically phosphorylates Y504 on LCK, which acts as a negative regulatory site. Csk and the phospho-tyrosine phosphatase CD45 reciprocally regulate phosphorylation of the inhibitory tyrosine of the Src family kinases Lck and Fyn during T-cell receptor mediated signaling during thymic development.

Protein type: Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Protein kinase, TK; Kinase, protein; TK group; Csk family

Chromosomal Location of Human Ortholog: 15q24.1

Cellular Component: cytoplasm; cytosol; extrinsic to internal side of plasma membrane; plasma membrane

Molecular Function: identical protein binding; kinase activity; non-membrane spanning protein tyrosine kinase activity; protein binding; protein C-terminus binding; protein-tyrosine kinase activity; receptor binding

Biological Process: cell differentiation; cell migration; central nervous system development; epidermal growth factor receptor signaling pathway; innate immune response; negative regulation of Golgi to plasma membrane protein transport; protein amino acid phosphorylation; regulation of cell proliferation; regulation of cytokine production; T cell costimulation; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway