CPE blocking peptide

CPE Antibody (C-term) Blocking Peptide

• Gene Names: CPE; CPH
• Synonyms: CPE; CPE Antibody (C-term) Blocking Peptide; Carboxypeptidase E; Carboxypeptidase H; CPH; Enkephalin convertase; Prohormone-processing carboxypeptidase; CPE blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human CPE. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 476

• Cellular Location: Isoform 1: Cytoplasmic vesicle, secretory vesicle membrane; Peripheral membrane protein. Secreted. Note: Associated with the secretory granule membrane through direct binding to lipid rafts in intragranular conditions.
• Tissue Location: Isoform 2, but not isoform 1, is overexpressed in hepatocellular carcinoma (at protein level), as well as in other tumors, including pheochromocytomas and paragangliomas
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for CPE blocking peptide

Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin.

NCBI and Uniprot Product Information

• NCBI GI #: 115892
• NCBI GeneID: 1363
• UniProt Accession #: P16870; Q9UIU9; A8K4N1; B3KR42; B4DFN4; D3DP33
• Molecular Weight: 49,886 Da
• NCBI Official Full Name: Carboxypeptidase E
• NCBI Official Synonym Full Names: carboxypeptidase E
• NCBI Official Symbol: CPE
• NCBI Official Synonym Symbols: CPH
• NCBI Protein Information: carboxypeptidase E
• UniProt Protein Name: Carboxypeptidase E
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPE
• UniProt Synonym Gene Names: CPE; CPH
• UniProt Entry Name: CBPE_HUMAN

NCBI Description

This gene encodes a member of the M14 family of metallocarboxypeptidases. The encoded preproprotein is proteolytically processed to generate the mature peptidase. This peripheral membrane protein cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. This protein may also function independently of its peptidase activity, as a neurotrophic factor that promotes neuronal survival, and as a sorting receptor that binds to regulated secretory pathway proteins, including prohormones. Mutations in this gene are implicated in type 2 diabetes. [provided by RefSeq, Nov 2015]

Uniprot Description

CPE: Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.17.10; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q32.3

Cellular Component: cell soma; extracellular space; Golgi apparatus; plasma membrane; secretory granule; secretory granule membrane

Molecular Function: carboxypeptidase activity; cell adhesion molecule binding; metallocarboxypeptidase activity; neurexin binding; serine carboxypeptidase activity

Biological Process: insulin processing; metabolic process; Wnt receptor signaling pathway