COT (MAP3K8/MEKK8) Blocking Peptide | MAP3K8 blocking peptide

COT (MAP3K8/MEKK8) Antibody (C-term) Blocking peptide

• Gene Names: MAP3K8; COT; EST; ESTF; TPL2; AURA2; MEKK8; Tpl-2; c-COT
• Synonyms: COT (MAP3K8/MEKK8); COT (MAP3K8/MEKK8) Antibody (C-term) Blocking peptide; Mitogen-activated protein kinase kinase kinase 8; Cancer Osaka thyroid oncogene; Proto-oncogene c-Cot; Serine/threonine-protein kinase cot; Tumor progression locus 2; TPL-2; MAP3K8; COT; ESTF; MAP3K8 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human MEKK8. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 467

• Cellular Location: Cytoplasm
• Tissue Location: Expressed in several normal tissues and human tumor-derived cell lines
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for MAP3K8 blocking peptide

Required for lipopolysaccharide (LPS)-induced, TLR4- mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF- alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B- cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.

NCBI and Uniprot Product Information

• NCBI GI #: 50403742
• NCBI GeneID: 1326
• UniProt Accession #: P41279; Q14275; Q5T855; Q9HC81; A8K2Q5; D3DRX1
• Molecular Weight: 49,620 Da
• NCBI Official Full Name: Mitogen-activated protein kinase kinase kinase 8
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 8
• NCBI Official Symbol: MAP3K8
• NCBI Official Synonym Symbols: COT; EST; ESTF; TPL2; AURA2; MEKK8; Tpl-2; c-COT
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 8
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 8
• Protein Family: Mitogen-activated protein kinase kinase kinase
• UniProt Gene Name: MAP3K8
• UniProt Synonym Gene Names: COT; ESTF; TPL-2
• UniProt Entry Name: M3K8_HUMAN

NCBI Description

This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]

Uniprot Description

Cot: an oncogenic Ser/Thr kinase of the STE group. Activates IkappaB kinases, thus inducing the nuclear translocation of NF-kappaB. Promotes the production of TNF-alpha and IL-2 during T lymphocyte activation. Interacts with NFkB-p105. Overexpressed and amplified in breast tumors. Viral insertions induce rat lymphomas and mouse mammary carcinomas. Isolated as a transforming factor in two cell lines. Mediates LPS activation of macrophages. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.25; Oncoprotein; Kinase, protein; Protein kinase, STE; STE group; STE-Unique family

Chromosomal Location of Human Ortholog: 10p11.23

Cellular Component: cytoplasm; cytosol

Molecular Function: MAP kinase kinase kinase activity; protein binding; protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: apoptosis; protein amino acid phosphorylation; stress-activated MAPK cascade; T cell costimulation

Disease: Lung Cancer