CHMP1A blocking peptide

CHMP1A Antibody (N-term) Blocking Peptide

• Gene Names: CHMP1A; PCH8; CHMP1; PRSM1; PCOLN3; VPS46A; VPS46-1
• Synonyms: CHMP1A; CHMP1A Antibody (N-term) Blocking Peptide; Charged multivesicular body protein 1a; Chromatin-modifying protein 1a; CHMP1a; Vacuolar protein sorting-associated protein 46-1; Vps46-1; hVps46-1; CHMP1A blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 196

• Cellular Location: Cytoplasm. Endosome membrane; Peripheral membrane protein. Nucleus matrix. Note: The cytoplasmic form is partially membrane-associated and localizes to early endosomes The nuclear form remains associated with the chromosome scaffold during mitosis. On overexpression, it localizes to nuclear bodies characterized by nuclease-resistant condensed chromatin
• Tissue Location: Expressed in placenta, cultured skin fibroblasts and in osteoblast cell line MG-63
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for CHMP1A blocking peptide

Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT- III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. May also be involved in chromosome condensation. Targets the Polycomb group (PcG) protein BMI1/PCGF4 to regions of condensed chromatin. May play a role in stable cell cycle progression and in PcG gene silencing.

NCBI and Uniprot Product Information

• NCBI GI #: 62510514
• NCBI GeneID: 5119
• UniProt Accession #: Q9HD42; Q14468; Q15779; Q96G31; A2RU09
• Molecular Weight: 7,363 Da
• NCBI Official Full Name: Charged multivesicular body protein 1a
• NCBI Official Synonym Full Names: charged multivesicular body protein 1A
• NCBI Official Symbol: CHMP1A
• NCBI Official Synonym Symbols: PCH8; CHMP1; PRSM1; PCOLN3; VPS46A; VPS46-1
• NCBI Protein Information: charged multivesicular body protein 1a
• UniProt Protein Name: Charged multivesicular body protein 1a
• Protein Family: Charged multivesicular body protein
• UniProt Gene Name: CHMP1A
• UniProt Synonym Gene Names: CHMP1a; Vps46-1; hVps46-1
• UniProt Entry Name: CHM1A_HUMAN

NCBI Description

This gene encodes a member of the CHMP/Chmp family of proteins which are involved in multivesicular body sorting of proteins to the interiors of lysosomes. The initial prediction of the protein sequence encoded by this gene suggested that the encoded protein was a metallopeptidase. The nomenclature has been updated recently to reflect the correct biological function of this encoded protein. Several transcripts encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]

Uniprot Description

CHMP1A: Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT- III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. May also be involved in chromosome condensation. Targets the Polycomb group (PcG) protein BMI1/PCGF4 to regions of condensed chromatin. May play a role in stable cell cycle progression and in PcG gene silencing. Belongs to the SNF7 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Protease

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: condensed nuclear chromosome; early endosome; endomembrane system; microtubule organizing center; nuclear matrix

Molecular Function: identical protein binding; metallopeptidase activity; protein binding; protein domain specific binding; protein homodimerization activity; zinc ion binding

Biological Process: cell division; cell separation during cytokinesis; gene silencing; mitotic chromosome condensation; mitotic metaphase plate congression; negative regulation of transcription by glucose; negative regulation of transcription, DNA-dependent; nuclear organization and biogenesis; vesicle-mediated transport

Disease: Pontocerebellar Hypoplasia, Type 8