CD4 blocking peptide

CD4 Antibody (N-term) Blocking Peptide

• Gene Names: CD4; CD4mut
• Synonyms: CD4; CD4 Antibody (N-term) Blocking Peptide; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD4 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human CD4. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 458

• Cellular Location: Cell membrane; Single-pass type I membrane protein Note: Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for CD4 blocking peptide

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.

NCBI and Uniprot Product Information

• NCBI GI #: 116013
• NCBI GeneID: 920
• UniProt Accession #: P01730; Q4ZGK2; Q5U066; Q9UDE5; B2R737; D3DUS5
• Molecular Weight: 51,111 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD4
• NCBI Official Synonym Full Names: CD4 molecule
• NCBI Official Symbol: CD4
• NCBI Official Synonym Symbols: CD4mut
• NCBI Protein Information: T-cell surface glycoprotein CD4
• UniProt Protein Name: T-cell surface glycoprotein CD4
• Protein Family: CD40 ligand
• UniProt Gene Name: CD4
• UniProt Entry Name: CD4_HUMAN

NCBI Description

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: early endosome; endoplasmic reticulum membrane; external side of plasma membrane; lipid raft; plasma membrane

Molecular Function: enzyme binding; glycoprotein binding; MHC class II protein binding; protein binding; protein homodimerization activity; protein kinase binding; receptor activity; transmembrane receptor activity; zinc ion binding

Biological Process: cell surface receptor linked signal transduction; entry into host cell; enzyme linked receptor protein signaling pathway; induction by virus of cell-cell fusion in host; maintenance of cellular protein localization; positive regulation of protein kinase activity; regulation of defense response to virus by virus; regulation of T cell activation; signal transduction; T cell costimulation; T cell differentiation; T cell receptor signaling pathway; T cell selection; viral envelope fusion with host membrane

Disease: Okt4 Epitope Deficiency