CD3EAP blocking peptide

CD3EAP (E474) Peptide

Cat. Num. AAA3004376

• Gene Names: POLR1G; ASE1; CAST; ASE-1; PAF49; RPA34; CD3EAP
• Applications: Blocking
• Purity: Synthetic peptide CD3EAP (E474). (Note: the amino acid sequence is proprietary).; The purity is >98%.
• Synonyms: CD3EAP; CD3EAP (E474) Peptide; DNA-directed RNA polymerase I subunit RPA34; A34.5; Antisense to ERCC-1 protein; ASE-1; CD3-epsilon-associated protein; CAST; CD3E-associated protein; RNA polymerase I-associated factor PAF49; ASE1; PAF49; CD3EAP blocking peptide

• Specificity: This peptide can be used with studies using MBS3004375 CD3EAP (E474) pAb.
• Purity/Purification: Synthetic peptide CD3EAP (E474). (Note: the amino acid sequence is proprietary).; The purity is >98%.
• Form/Format: 1 mg/ml in DI water.
• Sequence Length: 510

• Applicable Applications for CD3EAP blocking peptide: Blocking
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Related Product Information for CD3EAP blocking peptide

CD3EAP, also known as ASE1, CAST or PAF49, is a 510 amino acid protein that localizes to the nucleus and belongs to the eukaryotic RPA34 RNA polymerase subunit family. Existing as two alternatively spliced isoforms, CD3EAP functions as a component of the Pol I complex, specifically exhibiting DNA-dependent RNA polymerase activity and effectively catalyzing the the conversion of a nucleoside triphosphate into a diphosphate, thereby transcribing DNA into RNA. Isoform 2 of CD3EAP may be a component of the T cell receptor (TCR) complex and is subject to tyrosine phosphorylation in response to TCR stimulation. Both isoforms of CD3EAP are subject to DNA damage-dependent phosphorylation, probably by ATM or ATR.

NCBI and Uniprot Product Information

• NCBI GI #: 6912246
• NCBI GeneID: 10849
• NCBI Accession #: NP_036231.1
• NCBI GenBank Nucleotide #: NP_036231.1
• UniProt Accession #: O15446
• NCBI Official Full Name: DNA-directed RNA polymerase I subunit RPA34 isoform 2
• NCBI Official Synonym Full Names: RNA polymerase I subunit G
• NCBI Official Symbol: POLR1G
• NCBI Official Synonym Symbols: ASE1; CAST; ASE-1; PAF49; RPA34; CD3EAP
• NCBI Protein Information: DNA-directed RNA polymerase I subunit RPA34
• UniProt Protein Name: DNA-directed RNA polymerase I subunit RPA34
• UniProt Gene Name: CD3EAP
• UniProt Synonym Gene Names: ASE1; CAST; PAF49; ASE-1; CAST
• UniProt Entry Name: RPA34_HUMAN

Uniprot Description

ASE-1: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation. Belongs to the eukaryotic RPA34 RNA polymerase subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; Transcription initiation complex

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleolus; RNA polymerase I transcription factor complex; chromosome; nucleus; DNA-directed RNA polymerase I complex

Molecular Function: DNA-directed RNA polymerase activity

Biological Process: negative regulation of gene expression, epigenetic; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; gene expression; transcription initiation from RNA polymerase I promoter; termination of RNA polymerase I transcription; regulation of gene expression, epigenetic; transmembrane receptor protein tyrosine kinase signaling pathway; rRNA transcription