Rabbit anti-Human, Mouse CD3EAP Polyclonal Antibody | anti-CD3EAP antibody

CD3EAP Polyclonal Antibody

Cat. Num. AAA3010816

• Gene Names: POLR1G; ASE1; CAST; ASE-1; PAF49; RPA34; CD3EAP
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CD3EAP; Polyclonal Antibody; CD3EAP Polyclonal Antibody; ASE-1; ASE1; CAST; PAF49; anti-CD3EAP antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CD3EAP polyclonal antibody detects endogenous levels of CD3EAP protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 510

• Applicable Applications for anti-CD3EAP antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant protein of human CD3EAP
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using CD3EAP antibody.)

Related Product Information for anti-CD3EAP antibody

CD3EAP, also known as ASE1, CAST or PAF49, is a 510 amino acid protein that localizes to the nucleus and belongs to the eukaryotic RPA34 RNA polymerase subunit family. Existing as two alternatively spliced isoforms, CD3EAP functions as a component of the Pol I complex, specifically exhibiting DNA-dependent RNA polymerase activity and effectively catalyzing the the conversion of a nucleoside triphosphate into a diphosphate, thereby transcribing DNA into RNA. Isoform 2 of CD3EAP may be a component of the T cell receptor (TCR) complex and is subject to tyrosine phosphorylation in response to TCR stimulation. Both isoforms of CD3EAP are subject to DNA damage-dependent phosphorylation, probably by ATM or ATR.

NCBI and Uniprot Product Information

• NCBI GI #: 6912246
• NCBI GeneID: 10849
• NCBI Accession #: NP_036231.1
• NCBI GenBank Nucleotide #: NP_036231.1
• UniProt Accession #: O15446
• Molecular Weight: 70kDa
• NCBI Official Full Name: DNA-directed RNA polymerase I subunit RPA34 isoform 2
• NCBI Official Synonym Full Names: RNA polymerase I subunit G
• NCBI Official Symbol: POLR1G
• NCBI Official Synonym Symbols: ASE1; CAST; ASE-1; PAF49; RPA34; CD3EAP
• NCBI Protein Information: DNA-directed RNA polymerase I subunit RPA34
• UniProt Protein Name: DNA-directed RNA polymerase I subunit RPA34
• UniProt Gene Name: CD3EAP
• UniProt Synonym Gene Names: ASE1; CAST; PAF49; ASE-1; CAST
• UniProt Entry Name: RPA34_HUMAN

Uniprot Description

ASE-1: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation. Belongs to the eukaryotic RPA34 RNA polymerase subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; Transcription initiation complex

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleolus; RNA polymerase I transcription factor complex; chromosome; nucleus; DNA-directed RNA polymerase I complex

Molecular Function: DNA-directed RNA polymerase activity

Biological Process: negative regulation of gene expression, epigenetic; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; gene expression; transcription initiation from RNA polymerase I promoter; termination of RNA polymerase I transcription; regulation of gene expression, epigenetic; transmembrane receptor protein tyrosine kinase signaling pathway; rRNA transcription