CCM2 blocking peptide

CCM2 Blocking Peptide

Cat. Num. AAA9621590

• Gene Names: CCM2; OSM; C7orf22; PP10187
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: CCM2; CCM2 Blocking Peptide; C7orf22; Ccm2; CCM2 gene; CCM2_HUMAN; Cerebral cavernous malformation 2; Cerebral cavernous malformations 2 protein; Chromosome 7 open reading frame 22; Malcavernin; MGC4067; MGC4607; MGC74868; OSM; Osmosensing scaffold for MEKK3; OTTHUMP00000159554; OTTHUMP00000214270; OTTHUMP00000214271; OTTHUMP00000214273; PP10187; CCM2 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for CCM2 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for CCM2 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 71067341
• NCBI GeneID: 83605
• NCBI Accession #: NP_001025006.1
• NCBI GenBank Nucleotide #: NP_001025006.1
• UniProt Accession #: Q9BSQ5; Q71RE5; Q8TAT4; A4D2L4; B3KUV0; D3DVL4; E9PDJ3; F5H0E1; F5H551
• Molecular Weight: 42,073 Da
• NCBI Official Full Name: malcavernin isoform 1
• NCBI Official Synonym Full Names: cerebral cavernous malformation 2
• NCBI Official Symbol: CCM2
• NCBI Official Synonym Symbols: OSM; C7orf22; PP10187
• NCBI Protein Information: malcavernin; cerebral cavernous malformations 2 protein
• UniProt Protein Name: Malcavernin
• Protein Family: Cerebral cavernous malformations protein
• UniProt Gene Name: CCM2
• UniProt Synonym Gene Names: C7orf22
• UniProt Entry Name: CCM2_HUMAN

NCBI Description

This gene encodes a scaffold protein that functions in the stress-activated p38 Mitogen-activated protein kinase (MAPK) signaling cascade. The protein interacts with SMAD specific E3 ubiquitin protein ligase 1 (also known as SMURF1) via a phosphotyrosine binding domain to promote RhoA degradation. The protein is required for normal cytoskeletal structure, cell-cell interactions, and lumen formation in endothelial cells. Mutations in this gene result in cerebral cavernous malformations. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Nov 2009]

Uniprot Description

CCM2: Component of the CCM signaling pathway which is a crucial regulator of heart and vessel formation and integrity. May act through the stabilization of endothelial cell junctions. May function as a scaffold protein for MAP2K3-MAP3K3 signaling. Seems to play a major role in the modulation of MAP3K3- dependent p38 activation induced by hyperosmotic shock. Defects in CCM2 are the cause of cerebral cavernous malformations type 2 (CCM2). Cerebral cavernous malformations (CCMs) are congenital vascular anomalies of the central nervous system that can result in hemorrhagic stroke, seizures, recurrent headaches, and focal neurologic deficits. CCMs have an incidence of 0.1%-0.5% in the general population and are usually present clinically during the 3rd to 5th decades of life. The lesions are characterized by grossly enlarged blood vessels consisting of a single layer of endothelium and without any intervening neural tissue, ranging in diameter from a few millimeters to several centimeters. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: protein complex; cytoplasm

Molecular Function: protein binding

Biological Process: integrin-mediated signaling pathway; intercellular junction assembly and maintenance; pericardium development; venous blood vessel morphogenesis; in utero embryonic development; multicellular organism growth; stress-activated MAPK cascade; vasculogenesis; inner ear development; endothelial cell development

Disease: Cerebral Cavernous Malformations 2