CAPG blocking peptide

CAPG Blocking Peptide

Cat. Num. AAA9614552

• Gene Names: CAPG; MCP; AFCP; HEL-S-66
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: CAPG; CAPG Blocking Peptide; Actin capping protein GCAP39; Actin regulatory protein CAP G; Actin regulatory protein CAP-G; AFCP; Capg; CAPG_HUMAN; Capping actin protein gelsolin like; Capping protein (actin filament) gelsolin like; Capping protein gelsolin like; Epididymis secretory protein Li 66; gCap39; Gelsolin like capping protein; HEL S 66; Macrophage capping protein; Macrophage-capping protein; mbh1; MCP; Myc basic motif homolog 1; CAPG blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for CAPG blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for CAPG blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 313104088
• NCBI GeneID: 822
• UniProt Accession #: P40121; Q53SA7; B8ZZS7; D6W5K8
• Molecular Weight: 348
• NCBI Official Full Name: Macrophage-capping protein
• NCBI Official Synonym Full Names: capping protein (actin filament), gelsolin-like
• NCBI Official Symbol: CAPG
• NCBI Official Synonym Symbols: MCP; AFCP; HEL-S-66
• NCBI Protein Information: macrophage-capping protein; macrophage capping protein; gelsolin-like capping protein; actin regulatory protein CAP-G; actin-regulatory protein CAP-G; epididymis secretory protein Li 66
• UniProt Protein Name: Macrophage-capping protein
• Protein Family: Protein
• UniProt Gene Name: CAPG
• UniProt Synonym Gene Names: AFCP; MCP
• UniProt Entry Name: CAPG_HUMAN

NCBI Description

This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

CAPG: Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA. Belongs to the villin/gelsolin family.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p11.2

Cellular Component: F-actin capping protein complex; melanosome; nucleus

Molecular Function: actin binding

Biological Process: protein complex assembly; cell projection biogenesis; barbed-end actin filament capping