CAD blocking peptide

Phospho-CAD (Ser1859) Blocking Peptide

Cat. Num. AAA9616497

• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: CAD; Phospho-CAD (Ser1859) Blocking Peptide; Aspartate carbamoyltransferase; Aspartate transcarbamylase; CAD protein; CAD trifunctional protein; Carbamoyl phosphate synthetase 2; CPSase ATCase DHOase; Dihydroorotase; Glutamine dependent carbamoyl phosphate synthase; Multifunctional protein CAD; CAD blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for CAD blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for CAD blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 18105007
• NCBI GeneID: 790
• NCBI Accession #: NP_004332.2
• NCBI GenBank Nucleotide #: NM_004341.3
• UniProt Accession #: P27708; Q6P0Q0; D6W552
• Molecular Weight: 242,984 Da
• NCBI Official Full Name: CAD protein
• NCBI Official Synonym Full Names: carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
• NCBI Official Symbol: CAD
• NCBI Protein Information: CAD protein; CAD trifunctional protein; multifunctional protein CAD
• UniProt Protein Name: CAD protein
• Protein Family: Cadherin
• UniProt Gene Name: CAD
• UniProt Entry Name: PYR1_HUMAN

NCBI Description

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. [provided by RefSeq, Jul 2008]

Uniprot Description

CAD: a multifunctional enzyme that catalyzes four enzymatic activities of the pyrimidine pathway, including the rate-limiting first step. The four enzymatic activities involved in de novo pyrimidine synthesis are glutamine amidotransferase, carbamoyl-phosphate synthetase, aspartate transcarbamoylase, and dihydroorotase activities. Provides essential precursors for DNA synthesis. Required for the de novo synthesis of pyrimidine nucleotides and cell growth. Phosphorylation by p70S6K promotes CAD oligomerization, stimulating de novo synthesis of pyrimidines and progression through S phase in mammalian cells. Utilizes glutamine, bicarbonate, and aspartic acid to form a pyrimidine ring that is subsequently attached to ribose to generate a pyrimidine.

Protein type: EC 6.3.5.5; EC 2.1.3.2; Transferase; Ligase; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 3.5.2.3; Hydrolase; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 2p22-p21

Cellular Component: nucleoplasm; cell projection; protein complex; nuclear matrix; membrane; cell soma; plasma membrane; terminal button; nucleus; cytosol

Molecular Function: carbamoyl-phosphate synthase (ammonia) activity; identical protein binding; enzyme binding; zinc ion binding; carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity; aspartate carbamoyltransferase activity; ATP binding; protein kinase activity; dihydroorotase activity

Biological Process: lactation; organ regeneration; arginine biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; protein amino acid autophosphorylation; heart development; UTP biosynthetic process; peptidyl-threonine phosphorylation; glutamine metabolic process; response to testosterone stimulus; female pregnancy; liver development; response to caffeine; 'de novo' pyrimidine base biosynthetic process; response to cortisol stimulus; pyrimidine base metabolic process; pyrimidine nucleoside biosynthetic process; glutamine catabolic process; drug metabolic process; urea cycle; response to amine stimulus

Disease: Congenital Disorder Of Glycosylation, Type Iz