CA4 blocking peptide

CA4 Antibody (C-term) Blocking Peptide

• Gene Names: CA4; CAIV; Car4; RP17
• Synonyms: CA4; CA4 Antibody (C-term) Blocking Peptide; Carbonic anhydrase 4; Carbonate dehydratase IV; Carbonic anhydrase IV; CA-IV; CA4 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human CA4. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 312

• Cellular Location: Cell membrane; Lipid-anchor, GPI-anchor.
• Tissue Location: Expressed in the endothelium of the choriocapillaris in eyes (at protein level). Not expressed in the retinal epithelium at detectable levels
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for CA4 blocking peptide

Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid.

NCBI and Uniprot Product Information

• NCBI GI #: 115465
• NCBI GeneID: 762
• UniProt Accession #: P22748; Q6FHI7; B4DQA4
• Molecular Weight: 11,599 Da
• NCBI Official Full Name: Carbonic anhydrase 4
• NCBI Official Synonym Full Names: carbonic anhydrase 4
• NCBI Official Symbol: CA4
• NCBI Official Synonym Symbols: CAIV; Car4; RP17
• NCBI Protein Information: carbonic anhydrase 4
• UniProt Protein Name: Carbonic anhydrase 4
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA4
• UniProt Synonym Gene Names: CA-IV
• UniProt Entry Name: CAH4_HUMAN

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Its exact function is not known; however, it may have a role in inherited renal abnormalities of bicarbonate transport. [provided by RefSeq, Jul 2008]

Uniprot Description

CA4: Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid. Defects in CA4 are the cause of retinitis pigmentosa type 17 (RP17). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP17 inheritance is autosomal dominant. Defective acid overload removal from retina and retinal epithelium, due to mutant CA4, is responsible for photoreceptor degeneration, indicating that impaired pH homeostasis is the most likely cause underlying the RP17 phenotype. Belongs to the alpha-carbonic anhydrase family.

Protein type: Membrane protein, GPI anchor; Lyase; EC 4.2.1.1; Energy Metabolism - nitrogen

Chromosomal Location of Human Ortholog: 17q23

Cellular Component: anchored to external side of plasma membrane; apical plasma membrane; basolateral plasma membrane; brush border membrane; cell surface; ER-Golgi intermediate compartment; Golgi apparatus; membrane; perinuclear region of cytoplasm; plasma membrane; rough endoplasmic reticulum; secretory granule membrane; trans-Golgi network

Molecular Function: carbonate dehydratase activity; protein binding

Biological Process: bicarbonate transport

Disease: Retinitis Pigmentosa 17