BUB1 blocking peptide

BUB1 (F818) Peptide

Cat. Num. AAA3013773

• Gene Names: BUB1; BUB1A; BUB1L; hBUB1
• Applications: Blocking
• Purity: Synthetic peptide BUB1 (F818). (Note: the amino acid sequence is proprietary).; The purity is >98%.
• Synonyms: BUB1; BUB1 (F818) Peptide; Bub1; BUB1 budding uninhibited by benzimidazoles 1 homolog; BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast); BUB1A; BUB1L; Budding uninhibited by benzimidazoles 1 (yeast homolog); Budding uninhibited by benzimidazoles 1 homolog; hBUB1; Mitotic checkpoint serine/threonine protein kinase BUB1; Mitotic checkpoint serine/threonine-protein kinase BUB1; Mitotic spindle checkpoint kinase; Putative serine/threonine protein kinase; BUB1 blocking peptide

• Specificity: This peptide can be used with studies using MBS3013772 BUB1 (F818) pAb.
• Purity/Purification: Synthetic peptide BUB1 (F818). (Note: the amino acid sequence is proprietary).; The purity is >98%.
• Form/Format: 1 mg/ml in DI water.
• Sequence Length: 1085

• Applicable Applications for BUB1 blocking peptide: Blocking
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Related Product Information for BUB1 blocking peptide

Human cells contain two related protein kinases, BUB1 and BUBR1, that appear to have evolved from a single ancestral BUB1 gene. Both kinases are concentrated near the surface of the kinetochore where they monitor kinetochore-microtubule interactions. BUB1 and BUBR1 bind to kinetochores and are postulated to be components of the mitotic checkpoint, which monitors kinetochore activities to determine if chromosomes have achieved alignment at the spindle equator. BUBR1 is essential for normal mitotic progression as it prevents cells from prematurely entering anaphase. BUB3 is a conserved component of the mitotic spindle assembly complex and is also involved with the essential spindle checkpoint pathway that operates during early embryogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 4757878
• NCBI GeneID: 699
• NCBI Accession #: NP_004327.1
• NCBI GenBank Nucleotide #: NP_004327.1
• UniProt Accession #: O43683
• NCBI Official Full Name: mitotic checkpoint serine/threonine-protein kinase BUB1 isoform 1
• NCBI Official Synonym Full Names: BUB1 mitotic checkpoint serine/threonine kinase
• NCBI Official Symbol: BUB1
• NCBI Official Synonym Symbols: BUB1A; BUB1L; hBUB1
• NCBI Protein Information: mitotic checkpoint serine/threonine-protein kinase BUB1
• UniProt Protein Name: Mitotic checkpoint serine/threonine-protein kinase BUB1
• Protein Family: Checkpoint serine/threonine-protein kinase
• UniProt Gene Name: BUB1
• UniProt Synonym Gene Names: BUB1L; hBUB1
• UniProt Entry Name: BUB1_HUMAN

NCBI Description

This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

BUB1: a mitotic-checkpoint protein kinase of the BUB family. Autophosphorylated when the cells enters mitosis. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage. High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index. LOF mutations seen in two colon cancer cell lines exhibiting chromosomal instability, in several leukemias and lymphomas, and in a pancreatic cancer cell line. BUB1 functions in mitotic spindle assembly, and may cause chromosomal instability. In mouse BRCA2 knockouts, mutations in BUB1 and BUBR1 are frequently seen in the resulting tumors. BUB1 and BUBR1 are overexpressed, but not mutated, in many gastric cancers.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Cell cycle regulation; Other group; BUB family

Chromosomal Location of Human Ortholog: 2q14

Cellular Component: nucleoplasm; kinetochore; membrane; cytoplasm; cytosol; condensed nuclear chromosome, pericentric region

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity

Biological Process: cell proliferation; mitosis; viral reproduction; apoptosis; cell division; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; regulation of chromosome segregation; regulation of sister chromatid cohesion; mitotic cell cycle; protein amino acid phosphorylation; chromosome segregation