AWAT2 blocking peptide

AWAT2 Blocking Peptide

Cat. Num. AAA9628170

• Gene Names: AWAT2; WS; DC4; ARAT; MFAT; DGAT2L4
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: AWAT2; AWAT2 Blocking Peptide; Acyl CoA wax alcohol acyltransferase 2; Acyl-CoA retinol O-fatty-acyltransferase; Acyl-CoA wax alcohol acyltransferase 2; ARAT; Awat2; AWAT2_HUMAN; DC4; Diacylglycerol O acyltransferase 2 like 4; Diacylglycerol O acyltransferase candidate 4; Diacylglycerol O-acyltransferase 2-like protein 4; Diacylglycerol O-acyltransferase candidate 4; hDC4; hWS; Long chain alcohol O fatty acyltransferase 2; Long-chain-alcohol O-fatty-acyltransferase 2; MFAT; Multifunctional O acyltransferase; Multifunctional O-acyltransferase; Retinol O-fatty-acyltransferase; Wax synthase 2; Wax synthase; WS; AWAT2 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for AWAT2 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for AWAT2 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 51317389
• NCBI GeneID: 158835
• NCBI Accession #: NP_001002254.1
• NCBI GenBank Nucleotide #: NM_001002254.1
• UniProt Accession #: Q6E213; Q6IEE3; Q6P437
• Molecular Weight: 38,094 Da
• NCBI Official Full Name: acyl-CoA wax alcohol acyltransferase 2
• NCBI Official Synonym Full Names: acyl-CoA wax alcohol acyltransferase 2
• NCBI Official Symbol: AWAT2
• NCBI Official Synonym Symbols: WS; DC4; ARAT; MFAT; DGAT2L4
• NCBI Protein Information: acyl-CoA wax alcohol acyltransferase 2
• UniProt Protein Name: Acyl-CoA wax alcohol acyltransferase 2
• Protein Family: Acyl-CoA wax alcohol acyltransferase
• UniProt Gene Name: AWAT2
• UniProt Synonym Gene Names: DC4; DGAT2L4; MFAT; WS; ARAT; Retinol O-fatty-acyltransferase; hDC4; hWS
• UniProt Entry Name: AWAT2_HUMAN

NCBI Description

This gene encodes an enzyme belonging to the diacylglycerol acyltransferase family. This enzyme produces wax esters by the esterification of long chain (or wax) alcohols with acyl-CoA-derived fatty acids. It functions in lipid metabolism in the skin, mostly in undifferentiated peripheral sebocytes. This enzyme may also have acyl-CoA:retinol acyltransferase activities, where it catalyzes the synthesis of diacylglycerols and retinyl esters. [provided by RefSeq, May 2010]

Uniprot Description

DGAT2L4: Acyltransferase that predominantly esterify long chain (wax) alcohols with acyl-CoA-derived fatty acids to produce wax esters. Wax esters are enriched in sebum, suggesting that it plays a central role in lipid metabolism in skin. Has no activity using decyl alcohol and significantly prefers the C16 and C18 alcohols. May also have 2-acylglycerol O-acyltransferase (MGAT) and acyl- CoA:retinol acyltransferase (ARAT) activities, to catalyze the synthesis of diacylglycerols and retinyl esters; however this activity is unclear in vivo. Belongs to the diacylglycerol acyltransferase family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cofactor and Vitamin Metabolism - retinol; EC 2.3.1.75; EC 2.3.1.76; Lipid Metabolism - glycerolipid; Transferase

Chromosomal Location of Human Ortholog: Xq13.1

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: retinol O-fatty-acyltransferase activity; long-chain-alcohol O-fatty-acyltransferase activity

Biological Process: retinol metabolic process; wax biosynthetic process