ATP5E blocking peptide

ATP5E Blocking Peptide (C-Term)

• Gene Names: ATP5E; ATPE; MC5DN3
• Synonyms: ATP5E; ATP5E Blocking Peptide (C-Term); ATP synthase subunit epsilon; mitochondrial; ATPase subunit epsilon; ATP5E blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 41-51 of HUMAN ATP5E
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 51

• Cellular Location: Mitochondrion. Mitochondrion inner membrane.
• Tissue Location: Ubiquitous.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ATP5E blocking peptide

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 3023354
• NCBI GeneID: 514
• UniProt Accession #: P56381; Q53XU6; B2RDD0; E1P5H6
• Molecular Weight: 5,780 Da
• NCBI Official Full Name: ATP synthase subunit epsilon, mitochondrial
• NCBI Official Synonym Full Names: ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit
• NCBI Official Symbol: ATP5E
• NCBI Official Synonym Symbols: ATPE; MC5DN3
• NCBI Protein Information: ATP synthase subunit epsilon, mitochondrial
• UniProt Protein Name: ATP synthase subunit epsilon, mitochondrial
• UniProt Gene Name: ATP5E
• UniProt Synonym Gene Names: ATPase subunit epsilon
• UniProt Entry Name: ATP5E_HUMAN

NCBI Description

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the epsilon subunit of the catalytic core. Two pseudogenes of this gene are located on chromosomes 4 and 13. Read-through transcripts that include exons from this gene are expressed from the upstream gene SLMO2.[provided by RefSeq, Mar 2011]

Uniprot Description

ATP5E: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Defects in ATP5E are the cause of mitochondrial complex V deficiency nuclear type 3 (MC5DN3). MC5DN3 is a mitochondrial disorder with heterogeneous clinical manifestations including dysmorphic features, psychomotor retardation, hypotonia, growth retardation, cardiomyopathy, enlarged liver, hypoplastic kidneys and elevated lactate levels in urine, plasma and cerebrospinal fluid. Belongs to the eukaryotic ATPase epsilon family.

Protein type: Transporter; Membrane protein, integral; Hydrolase; Mitochondrial; EC 3.6.3.14; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: 20q13.32

Cellular Component: mitochondrial matrix; mitochondrial proton-transporting ATP synthase complex

Molecular Function: ATPase activity; transmembrane transporter activity

Biological Process: ATP biosynthetic process; mitochondrial ATP synthesis coupled proton transport

Disease: Mitochondrial Complex V (atp Synthase) Deficiency, Nuclear Type 3