ATG4A blocking peptide

ATG4A Peptide

Cat. Num. AAA153925

• Gene Names: ATG4A; APG4A; AUTL2
• Applications: Blocking (BL)
• Synonyms: ATG4A; ATG4A Peptide; Autophagy related 4A cysteine peptidase; APG4-A; AUTL2; autophagy related 4A; cysteine peptidase; ATG4A blocking peptide

• Form/Format: Liquid
• Concentration: 200 ug/mL (varies by lot)
• Sequence Length: 398

• Applicable Applications for ATG4A blocking peptide: Blocking (BL)
• Application Notes: ATG4A peptide is used for blocking the activity of ATG4A antibody.
• Species: Human
• Buffer: PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide
• Location: 18 amino acid peptide near the carboxy terminus of human ATG4A.
• Preparation and Storage: Store ATG4A peptide at -20 degree C, stable for one year.

NCBI and Uniprot Product Information

• NCBI GI #: 30795252
• NCBI GeneID: 115201
• NCBI Accession #: NP_443168
• NCBI GenBank Nucleotide #: NM_052936.3
• UniProt Accession #: Q8WYN0; O95534; Q5JYY9; Q5JYZ0; Q86VE5; Q96KQ0; Q96KQ1; A6NCH2; B2RAZ7; D3DUY0
• Molecular Weight: 42,453 Da
• NCBI Official Full Name: cysteine protease ATG4A isoform a
• NCBI Official Synonym Full Names: autophagy related 4A, cysteine peptidase
• NCBI Official Symbol: ATG4A
• NCBI Official Synonym Symbols: APG4A; AUTL2
• NCBI Protein Information: cysteine protease ATG4A; hAPG4A; autophagin 2; autophagin-2; APG4 autophagy 4 homolog A; AUT-like 2 cysteine endopeptidase; ATG4 autophagy related 4 homolog A; AUT-like 2, cysteine endopeptidase; autophagy-related protein 4 homolog A; autophagy-related cysteine endopeptidase 2
• UniProt Protein Name: Cysteine protease ATG4A
• Protein Family: Cysteine protease
• UniProt Gene Name: ATG4A
• UniProt Synonym Gene Names: APG4A; AUTL2; hAPG4A
• UniProt Entry Name: ATG4A_HUMAN

NCBI Description

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Transcript variants that encode distinct isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

ATG4A: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver. Inhibited by N-ethylmaleimide. Belongs to the peptidase C54 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Microtubule-binding; Protease; EC 3.4.22.-

Chromosomal Location of Human Ortholog: Xq22.3

Cellular Component: cytoplasm; cytosol

Molecular Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: protein delipidation; mitochondrion degradation; C-terminal protein lipidation; protein processing; proteolysis; protein targeting to membrane; cellular response to nitrogen starvation; autophagic vacuole formation